18 results on '"Tazuma, Susumu"'
Search Results
2. Partial Characterization of Mechanisms of Cytoprotective Action of Hydrophilic Bile Salts Against Hydrophobic Bile Salts in Rats: Relation to Canalicular Membrane Fluidity and Packing Density
- Author
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MIYAKE, HIROAKI, TAZUMA, SUSUMU, MIURA, HIROYUKI, YAMASHITA, GUNJI, and KAJIYAMA, GORO
- Published
- 1999
3. Comparing the acid-suppressive effects of three brands of generic lansoprazole with the original: pharmacokinetic bioequivalence tests do not necessarily guarantee pharmacodynamic equivalence.
- Author
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Shimatani, Tomohiko, Hirokawa, Seiko, Tawara, Yumiko, Hamai, Kazuko, Matsumoto, Mutsuko, Tazuma, Susumu, and Inoue, Masaki
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LANSOPRAZOLE ,GENERIC drugs ,THERAPEUTIC equivalency in drugs ,GENERIC drug substitution ,HELICOBACTER pylori ,COMPARATIVE studies ,HYDROGEN-ion concentration ,RESEARCH methodology ,MEDICAL cooperation ,OXIDOREDUCTASES ,RESEARCH ,STOMACH ,SULFUR compounds ,PROTON pump inhibitors ,EVALUATION research - Abstract
Generic drugs contain the same active ingredient as an original drug and have their bioequivalence proved by pharmacokinetic tests. However, few studies have been reported on whether these bioequivalence studies infer pharmacodynamic equivalence. In this study, in eight healthy Helicobacter pylori-negative CYP2C19 extensive metabolizers, we compared the acid-suppressive effects of repeated administration of 15 mg of three brands of generic lansoprazole, Taiproton, Tapizol, and Lansoral, with those of the original lansoprazole, Takepron. Median intragastric pH value for 24-h and % pH > 4 for daytime (08:00-20:00 h) and night-time were significantly higher with any lansoprazole formulation, compared with the control (P < 0.05, Wilcoxon signed-rank test). However, during the daytime, % pH > 4 with Tapizol was significantly lower than the original (P < 0.05). Compared with the original, no significantly larger, but no small range of inter-subject variations were observed in these two parameters for each of the three brands of generic lansoprazole (Bartlett test). Pharmacokinetic bioequivalence tests do not necessarily guarantee pharmacodynamic equivalence. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
4. Lafutidine, a newly developed antiulcer drug, elevates postprandial intragastric pH and increases plasma calcitonin gene-related peptide and somatostatin concentrations in humans: comparisons with famotidine.
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Shimatani, Tomohiko, Inoue, Masaki, Kuroiwa, Tomoko, Xu, Jing, Nakamura, Masuo, Tazuma, Susumu, Ikawa, Kazuro, and Morikawa, Norifumi
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ACETIC acid ,COMPARATIVE studies ,CROSSOVER trials ,GASTRIC acid ,HYDROGEN-ion concentration ,IMMUNOENZYME technique ,INGESTION ,LONGITUDINAL method ,RESEARCH methodology ,MEDICAL cooperation ,NEUROPEPTIDES ,ORAL drug administration ,PIPERIDINE ,PYRIDINE ,REFERENCE values ,RESEARCH ,SOMATOSTATIN ,STOMACH ,EVALUATION research ,RANDOMIZED controlled trials ,FAMOTIDINE ,GASTRIC acidity determination ,H2 receptor antagonists ,PHARMACODYNAMICS - Abstract
Lafutidine, a newly developed histamine H(2)-receptor antagonist, inhibits daytime (i.e., postprandial) as well as nighttime gastric acid secretion in clinical studies. It also has gastroprotective activity that particularly affects mucosal blood flow in rats. This study focused on the efficacy of lafutidine on plasma concentrations of gastrointestinal peptides in humans. Six healthy male volunteers aged 23-32 years without Helicobacter pylori infection were orally administered either 10 mg lafutidine, 20 mg famotidine, or water only (control) 30 min after a standard meal (650 kcal). Plasma concentrations of lafutidine and famotidine were highest from 90 to 150 min after administration. Intragastric pH was elevated after both lafutidine and famotidine compared with the control. Plasma concentrations of calcitonin gene-related peptide (CGRP) and somatostatin were significantly increased after lafutidine at 60 and 90 min. We concluded that lafutidine increases plasma concentrations of CGRP and somatostatin in humans, which may result in inhibition of postprandial acid secretion and gastroprotective activity. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
5. Hepatitis C virus core protein modulates fatty acid metabolism and thereby causes lipid accumulation in the liver.
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Yamaguchi, Atsushi, Tazuma, Susumu, Nishioka, Tomoji, Ohishi, Waka, Hyogo, Hideyuki, Nomura, Shuichi, and Chayama, Kazuaki
- Subjects
PROTEIN metabolism ,ANIMAL experimentation ,CELL lines ,FATTY acids ,FATTY liver ,GENES ,GENETIC disorders ,GENETIC techniques ,LIPID metabolism disorders ,LIVER ,MICE ,PROTEINS ,TRIGLYCERIDES - Abstract
We studied the roles of hepatitis C virus (HCV) core protein in hepatic steatosis and changes in hepatic lipid metabolism. HCV core protein expression plasmid was transfected in HepG2. Triacylglyceride (TG) and mRNA level associated with lipid metabolism were measured. Male C57BL/6 mice were infected with HCV core recombinant adenovirus and used for lipids and mRNA studies. In HCV core protein-expressing cells, peroxisome proliferator-activated receptor (PPAR)alpha, multidrug resistance protein (MDR) 3, and microsomal triglyceride transfer protein (MTP) were down-regulated 48 hr after transfection. In HCV core protein-expressing mice, hepatic TG content and hepatic thiobarbituric acid-reactive substances increased. PPARalpha, MDR2, acyl-CoA oxidase (AOX), and carnitine palmitoyl transferase-1 (CPT-1) were down-regulated. HCV core protein down-regulated lipid metabolism-associated gene expression, Mdr2, CPT, and AOX, accompanied by down-regulation of PPARalpha. There findings may contribute to the understanding of HCV-related steatosis, induction of reactive oxygen species, and carcinogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2005
6. Acid-suppressive efficacy of a reduced dosage of rabeprazole: comparison of 10 mg twice daily rabeprazole with 20 mg twice daily rabeprazole, 30 mg twice daily lansoprazole, and 20 mg twice daily omeprazole by 24-hr intragastric pH-metry.
- Author
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Shimatani, Tomohiko, Inoue, Masaki, Kuroiwa, Tomoko, Xu, Jing, Tazuma, Susumu, Horikawa, Yoko, and Nakamura, Masuo
- Abstract
Rabeprazole achieves more potent acid suppression than other proton pump inhibitors. Therefore it is administered at reduced as well as high dosages in eradication therapy for Helicobacter pylori; however, there is incomplete assessment of the efficacy of a reduced dosage of rabeprazole as might be employed in therapy. In this study, we evaluated acid-suppressive efficacy of a reduced dosage of rabeprazole on day 7 by 24-hr pH-metry in 10 healthy male cytochrome P-450 2C19 extensive metabolizers without Helicobacterpylori infection and compared the results with those of high dosages of rabeprazole, lansoprazole, and omeprazole. Median intragastric pH value, pH >3 holding time ratio (pH>3HT), pH>4HT, pH>5HT, pH>6HT, and pH>7HT for 24 hr with rabeprazole, 10 mg twice daily, were not significantly different from those of rabeprazole, 20 mg twice daily, lansoprazole, 30 mg twice daily, and omeprazole, 20 mg twice daily. In conclusion, for acid-suppressive efficacy, a reduced dosage of rabeprazole is comparable to high dosages of rabeprazole, lansoprazole, and omeprazole. [ABSTRACT FROM AUTHOR]
- Published
- 2005
7. Angiotensin II participates in hepatic inflammation and fibrosis through MCP-1 expression.
- Author
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Kanno, Keishi, Tazuma, Susumu, Nishioka, Tomoji, Hyogo, Hideyuki, and Chayama, Kazuaki
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In this study, we assessed the hypothesis that angiotensin (Ang) II could modulate inflammatory cell recruitment into the liver through hepatic expression of monocyte chemoattractant protein (MCP)-1 during liver injury. For in vivo study, Ang II type la knockout (ATla KO) mice and wild-type (WT) mice were treated with CCl4 for 4 weeks. After CCl4 treatment, ATla KO mice showed lower expression of MCP-1 and fewer CD68-positive cells in the liver compared with WT mice. For in vitro study, Ang II was added to LI90 cells. Ang II enhanced MCP-1 mRNA together with RhoA mRNA and also induced secretion of MCP-1 into the culture medium. This change was strongly blocked by Y-27632, a specific Rho-kinase inhibitor. These results suggest that Ang II modulates hepatic inflammation via production of MCP-1 by hepatic stellate cells, and the effect of Ang II on MCP-1 production is, at least partly, mediated by the Rho/Rho-kinase pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2005
8. A nuclear receptor ligand down-regulates cytosolic phospholipase A2 expression to reduce bile acid-induced cyclooxygenase 2 activity in cholangiocytes: implications of anticarcinogenic action of farnesoid X receptor agonists.
- Author
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Komichi, Daisuke, Tazuma, Susumu, Nishioka, Tomoji, Hyogo, Hideyuki, and Chayama, Kazuaki
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ANIMAL experimentation ,BILIARY tract ,BIOCHEMISTRY ,CELL culture ,CELL receptors ,COMPARATIVE studies ,DOCUMENTATION ,GENES ,PHENOMENOLOGY ,RESEARCH methodology ,MEDICAL cooperation ,MICE ,NUCLEOTIDES ,OXIDOREDUCTASES ,POLYMERASE chain reaction ,PROBABILITY theory ,RESEARCH ,WESTERN immunoblotting ,EVALUATION research ,REVERSE transcriptase polymerase chain reaction - Abstract
Bile acids are considered to be involved in the development of biliary tract carcinoma, although the underlying mechanisms are yet to be established. The aims of this study were (1) to investigate the carcinogenic role of bile acids in the biliary system based on the arachidonate-prostanoid pathway and (2) to clarify the therapeutic role of a farnesoid X receptor (FXR) ligand that modifies bile acid metabolism. Immortalized mouse cholangiocytes were incubated with glycochenodeoxycholate (GCDC), taurocholate, taurochenodeoxycholate, taurodeoxycholate, and tauroursodeoxycholate. GCDC induced cyclooxygenase 2 (COX-2) expression (Western blotting, 1.7-fold; RT-PCR, 2.3-fold) and prostaglandin (PG) production (PGE2, 6.3-fold; PGF2alpha, 8.5-fold), whereas cytosolic phospholipase A2 (cPLA2) expression and activity were reduced. In contrast, no marked changes were induced by the other bile acids. When the same experiment was performed in the presence of a synthetic FXR ligand (GW4064), cPLA2 expression and activity were reduced, although COX-2 expression was unchanged. GW4064 also suppressed PG generation by 40%. In conclusion, the present findings suggest a carcinogenic potential of GCDC. A synthetic FXR ligand (GW4064) inhibited the induction of COX-2 activity (detected as PG production) by GCDC, suggesting its anticarcinogenic potential. This effect seemed to be due to down-regulation of cPLA2. FXR ligands may have therapeutic potential against biliary carcinogenesis, but a delivery system for these agents is still to be developed. [ABSTRACT FROM AUTHOR]
- Published
- 2005
9. Gastric acid normosecretion is not essential in the pathogenesis of mild erosive gastroesophageal reflux disease in relation to Helicobacter pylori status.
- Author
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Shimatani, Tomohiko, Inoue, Masaki, Harada, Nobue, Horikawa, Yoko, Nakamura, Masuo, and Tazuma, Susumu
- Abstract
In the pathogenesis of gastroesophageal reflux disease (GERD), gastric acid is considered to be one of the most important factors, but little is known about the degree of gastric acid secretion in GERD patients. In this study, we evaluated it in GERD patients and control subjects by 24-h intragastric pH, and serological and histological investigations, in relation to Helicobacter pylori (H. pylori) status. In H. pylori-negative GERD patients gastric acid secretion was similar to that in H. pylori-negative control subjects. In H. pylori-positive GERD patients, in particular, mild GERD patients, it decreased significantly compared to that in H. pylori-negative control subjects, but the degree of decrease was smaller than in H. pylori-positive control subjects. Results of serological and histological evaluation were supportive. In conclusion, in some GERD patients, gastric acid secretion was significantly decreased. Increased or maintained gastric acid secretion was not essential in the pathogenesis of mild GERD. [ABSTRACT FROM AUTHOR]
- Published
- 2004
10. Unique inhibition of bile salt-induced apoptosis by lecithins and cytoprotective bile salts in immortalized mouse cholangiocytes.
- Author
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Komichi, Daisuke, Tazuma, Susumu, Nishioka, Tomoji, Hyogo, Hideyuki, Une, Mizuho, and Chayama, Kazuaki
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BILE duct diseases ,BILE salts ,APOPTOSIS ,LECITHIN ,EPITHELIUM ,MULTIDRUG resistance ,ANIMAL experimentation ,BILE ducts ,CARRIER proteins ,COMPARATIVE studies ,DNA probes ,DOSE-effect relationship in pharmacology ,FLOW cytometry ,RESEARCH methodology ,MEDICAL cooperation ,MICE ,POLYMERASE chain reaction ,PROTEINS ,PROTEOLYTIC enzymes ,RADIOISOTOPES ,RESEARCH ,WESTERN immunoblotting ,EVALUATION research ,REVERSE transcriptase polymerase chain reaction ,ION transport (Biology) - Abstract
Bile duct epithelium is physiologically exposed to high concentrations of bile salts, suggesting the presence of a cytoprotective mechanism(s). The aim of this study was to clarify whether bile salts cause bile duct cell damage and to elucidate the mechanism(s) providing protection against such an action of bile salts. Immortalized mouse cholangiocytes were incubated with taurocholate, taurochenodeoxycholate, glycochenodeoxycholate (GCDC), taurodeoxycholate, and tauroursodeoxycholate (TUDC), followed by flow-cytometric analysis and caspase activity assay to evaluate the induction of apoptosis. GCDC time-dependently induced caspase 3 (3.4-fold)- and caspase 9 (1.4-fold)-mediated apoptosis of cholangiocytes, but this was inhibited by lecithins and TUDC. Further, expression of cholangiocyte bile salt transporters (apical sodium-dependent bile salt transporter [Asbt] and multidrug resistance protein 3 [Mrp3]) was examined by RT-PCR and western blotting, and cholangiocyte bile salt uptake was determined using radiolabeled bile salts. Expression of cholangiocyte Asbt and Mrp3 was increased by bile salts, whereas lecithins interestingly reduced bile salt uptake to inhibit cholangiocyte apoptosis. In conclusion, bile salts themselves cause cholangiocyte apoptosis when absorbed by and retained inside the cell, but this is inhibited by washing out cytotoxic bile salts according to Mrp3, a rescue exporting molecule. Biliary lecithin is seemingly another cytoprotective player against cytotoxic bile salts, reducing their uptake, and this is associated with a reduced expression of Mrp3. [ABSTRACT FROM AUTHOR]
- Published
- 2003
11. Phospholipid alterations in hepatocyte membranes and transporter protein changes in cholestatic rat model.
- Author
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Hyogo, Hideyuki, Tazuma, Susumu, Nishioka, Tomoji, Ochi, Hidenori, Yamaguchi, Atushi, Numata, Yoshihiro, Kanno, Keishi, Sakomoto, Minoru, Asamoto, Yasumasa, Tsuboi, Kazuhiko, Nakai, Kuniharu, Yasumiba, Shigeyuki, Sunami, Yasushi, Kajiyama, Goro, Hyogo, H, Tazuma, S, Nishioka, T, Ochi, H, Yamaguchi, A, and Numata, Y
- Abstract
Biliary components are transported by hepatic adenosine triphosphate-binding cassette (ABC) transporters that are located in canalicular membranes. Physiological transporter function is related to membrane fluidity, which is modulated by the phospholipid composition of the lipid bilayer. We hypothesized that cholestasis may alter transporter function by modifying phospholipid species to protect the cell from cholestatic damage. Therefore, we examined the expression of ABC transport proteins and their mRNA levels in canalicular membrane vesicles isolated from rat liver 6 hr or three days after bile duct ligation. Membrane lipid composition and membrane fluidity of both sinusoidal and canalicular membrane vesicles were also examined. By 6 hr after bile duct ligation, we found a clear increase of mdr2 and bsep mRNA. These changes were associated with an increase of mdr-Pgp and with a clear decrease of mrp2 protein, and small decrease of bsep protein. In addition, mdrlb mRNA showed a strong increase by three days after bile duct ligation. Canalicular membrane fluidity decreased in a marked time-dependent manner, whereas sinusoidal membranes showed biphasic changes: increased fluidity at 6 hr and a decrease at three days. These changes were closely related to the changes of membrane lipid constitution; the saturated/unsaturated fatty acid ratio increased for phosphatidylcholine in canalicular membrane and the reverse occurred in sinusoidal membrane, and those for sphingomyelin showed the opposite pattern. We conclude that cholestasis causes modulation of ABC transporters as well as that of the lipid constitution in lipid bilayer. These may confer cytoprotective resistance to hepatocytes against cholestatic stress. [ABSTRACT FROM AUTHOR]
- Published
- 2001
- Full Text
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12. Bile acid reflux and possible inhibition of Helicobacter pylori infection in subjects without gastric surgery.
- Author
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Kawai, Yukinobu, Tazuma, Susumu, Inoue, Masaki, Kawai, Y, Tazuma, S, and Inoue, M
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AMMONIA analysis ,BILIOUS diseases & biliousness ,DUODENAL ulcers ,GASTRIC acid ,HELICOBACTER diseases ,HELICOBACTER pylori ,PEPTIC ulcer ,GASTRIC acidity determination ,DISEASE complications - Abstract
Bile acids are generally known to inhibit growth of Helicobacter pylori in vitro, but whether they do so in humans with no gastric surgery has been uncertain. The present study addresses this issue. Among healthy control subjects with preserved acid secretion, H. pylori-positive subjects were older and had lower gastric bile acid concentrations than H. pylori-negative subjects (P < 0.05). Among gastric ulcer patients with preserved acid secretion, H. pylori-positive patients had a higher basal acid output than H. pylori-negative patients (P < 0.05). Among H. pylori-positive subjects with preserved acid secretion, duodenal ulcer patients had a higher basal and maximum acid output than healthy control subjects (P < 0.01). In conclusion, gastric bile acids may suppress initial stages of H. pylori infection in subjects without gastric surgery. However gastric bile acids may have little effect on peptic ulcer disease, once H. pylori infection is established. [ABSTRACT FROM AUTHOR]
- Published
- 2001
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13. Modifying hepatic phospholipid synthesis associates with biliary phospholipid secretion rate in a transporter-independent manner in rats: relation to canalicular membrane fluidity.
- Author
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Yasumiba, Shigeyuki, Tazuma, Susumu, Ochi, Hidenori, Kajiyama, Goro, Yasumiba, S, Tazuma, S, Ochi, H, Kajiyama, G, and Mdt
- Abstract
Biliary phospholipid secretion is mediated by a multidrug resistance gene product, and its molecular subselection occurs at the site of secretion to modulates bile metastability. The aim of this study was to determine the effect of modifying hepatic phospholipid synthesis on canalicular phospholipid transporter expression and membrane fluidity. Bile-duct cannulation was performed in male Sprague-Dawley rats pretreated with or without intravenous infusion of dimethylethanolamine, an intermediate phospholipid metabolite along the pathway of phosphatidylcholine synthesis of phosphatidylethanolamine N-methylation (0.01 mg/min/100 g body wt) for 15 hr, followed by sodium taurocholate infusion (50 nmol/min/100 g body wt) with or without sulfobromophthalein (50 nmol/min/100 g body wt). Dimethylethanolamine enhanced biliary phospholipid secretion in association with a decrease in biliary phospholipid hydrophobicity. Dimethylethanolamine also increased canalicular membrane fluidity defined by 1,6-diphenyl-1,3,5-hexatriene fluorescence depolarization, whereas the expression of multidrug resistance gene product and multidrug resistance associated protein was unchanged. In contrast, a disproportionate reduction of biliary phospholipid secretion caused by sulfobromophthalein (uncoupling) was enhanced by under the treatment with dimethylethanolamine. In conclusion, the increase in biliary phospholipid secretion and canalicular membrane fluidity without a drastic change of its canalicular transporter by dimethylethanolamine suggests that such a canalicular membrane fluidity facilitates the transporter activity and/or phospholipid molecular movement from the canalicular outer membrane into the bile. A more drastic reduction in phospholipid secretion under sulfobromophthalein-caused uncoupling indicates the possibility of a preferential distribution of relatively hydrophilic phosphatidylcholine molecules to bile salt micelles since sulfobromophthalein is known to reduce the micellar capacity to extract membrane lipids for biliary secretion. [ABSTRACT FROM AUTHOR]
- Published
- 2001
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14. Dose-dependent conjugation of sulfobromophthalein and hepatic transit time in bile fistula rats: role of the microtubule-dependent vesicle pathway.
- Author
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Tazuma, Susumu, Horikawa, Kazuhiko, Ochi, Hidenori, Nishioka, Tomoji, Sunami, Yasushi, Yasumiba, Shigeyuki, Asamoto, Yasumasa, Tsuboi, Kazuhiko, Nakai, Kuniharu, Sakomoto, Minoru, Kanno, Keishi, Yamaguchi, Atsushi, Numata, Yoshihiro, Chayama, Kazuaki, Tazuma, S, Horikawa, K, Ochi, H, Nishioka, T, Sunami, Y, and Yasumiba, S
- Subjects
ANIMAL experimentation ,BILE ducts ,CHEMICAL reagents ,COLCHICINE ,COMPARATIVE studies ,CYTOPLASM ,DOSE-effect relationship in pharmacology ,LIVER ,RESEARCH methodology ,MEDICAL cooperation ,RATS ,RESEARCH ,TIME ,EVALUATION research - Abstract
Sulfobromophthalein (BSP) is selectively taken up by the liver and secreted into the bile as unconjugated and conjugated forms. Our previous study demonstrated that unconjugated BSP, but not conjugated BSP, caused the dissociation of biliary lipid secretion from that of bile acids, suggesting that the hepatic BSP conjugation rate partly regulated biliary lipid secretion. To evaluate the mechanisms through which biliary lipid secretion is regulated by exogenous organic anions, we intravenously administered BSP to male Sprague-Dawley rats at various doses either continuously or as a bolus. Then the relationship of the dose of BSP to its conjugation rate, hepatic transit time, and biliary lipid secretion was determined. BSP decreased biliary secretion of cholesterol and phospholipids in a dose-dependent manner without affecting bile acid secretion. In contrast, the proportion of conjugated BSP in bile was associated with the dose. Although the serum clearance of BSP after bolus infusion was constant regardless of the dose administered (50 or 200 nmol/100 g), BSP secretion was delayed with increasing doses: unconjugated BSP was secreted predominantly in the early phase (0-15 min after bolus injection), and conjugated BSP was the predominant form in the late phase (15-30 min). Pretreatment with colchicine reduced the conjugation rate and hepatic transit time of BSP, suggesting that the microtubule-dependent vesicle pathway plays a role in biliary excretion and conjugation of BSP. We conclude that biliary lipid secretion is influenced by organic anions with an affinity for bile acids such as BSP and that this effect is dependent upon the hepatic metabolic rate, i.e., conjugation rate. The hepatic transit time also plays a key role in this process by influencing metabolism. [ABSTRACT FROM AUTHOR]
- Published
- 2001
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15. Gallbladder dysfunction enhances physical density but not biochemical metastability of biliary vesicles.
- Author
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Sunami, Yasushi, Tazuma, Susumu, Kajiyama, Goro, Sunami, Y, Tazuma, S, and Kajiyama, G
- Abstract
The gallbladder role in cholesterol gallstone pathogenesis occurs through modulation of bile cholesterol metastability. The present study characterized the effects of concentrating bile on cholesterol crystallization through vesicle transformation, crystal habits, and potentiation of effector substances. Supersaturated model biles with total lipid concentrations of 12, 9, 6, and 3 g/dl were prepared with identical molar ratios (taurocholate-egg yolk phosphatidylcholine-cholesterol: 71:18:11). Bile metastability was assessed spectrophotometrically, and morphology of vesicle and crystal was sequentially scanned by video-enhanced differential contrast microscopy. The effects of replacing 30% of egg yolk phosphatidylcholine with soy bean phosphatidylcholine, 30% of taurocholate with taurodeoxycholate or tauroursodeoxycholate, and addition of concanavalin A-binding glycoprotein on each model bile were examined. By lowering total lipid concentration, cholesterol crystallization was retarded with less fusion and aggregation of vesicles. The effects of substances promoting cholesterol crystallization were enhanced with lesser bile. By replacing 30% of taurocholate with tauroursodeoxycholate, cholesterol crystallization was markedly inhibited in all concentrations, forming stable liquid-crystals. Impaired water absorption by the gallbladder may stabilize vesicles and inhibit rapid cholesterol crystallization, but the potential of cholesterol crystallization effector substances must be modified to alter bile cholesterol metastability. [ABSTRACT FROM AUTHOR]
- Published
- 2000
- Full Text
- View/download PDF
16. Role of phospholipase A2 in cholesterol gallstone formation is associated with biliary phospholipid species selection at the site of hepatic excretion: indirect evidence.
- Author
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Hattori, Yoshihiro, Tazuma, Susumu, Yamashita, Gunji, Ochi, Hidenori, Sunami, Yasushi, Nishioka, Tomoji, Hyogo, Hideyuki, Yasumiba, Shigeyuki, Kajihara, Tsuyoshi, Nakai, Kuniharu, Tsuboi, Kazuhiko, Asamoto, Yasumasa, Sakomoto, Minoru, Kajiyama, Goro, Hattori, Y, Tazuma, S, Yamashita, G, Ochi, H, Sunami, Y, and Nishioka, T
- Subjects
PROTEIN analysis ,LIPID analysis ,EGGS ,BILE ,CHOLESTEROL ,COMPARATIVE studies ,CRYSTALLIZATION ,CRYSTALLOGRAPHY ,ESTERASES ,GALLSTONES ,LECITHIN ,LIVER ,RESEARCH methodology ,MEDICAL cooperation ,MICROSCOPY ,PHOSPHOLIPIDS ,RESEARCH ,TELEVISION ,EVALUATION research - Abstract
Phospholipase A2 plays a role in cholesterol gallstone development by hydrolyzing bile phospholipids into lysolecithin and free fatty acids. Lysolecithin and polyunsaturated free fatty acids are known to stimulate the synthesis and/or secretion of gallbladder mucin via a prostanoid pathway, leading to enhancing cholesterol crystal nucleation and growth, and therefore, the action of phospholipase A2 is associated, in part, with bile phospholipid fatty acid. To clarify this hypothesis, we evaluated the effect on bile lipid metastability in vitro of replacing phospholipids with lysolecithin and various free fatty acids. Supersaturated model biles were created with an identical composition (cholesterol saturation index, 1.8; egg yolk lecithin, 34 mM; taurocholate, 120 mM; cholesterol, 25 mM) except for 5%, 10%, or 20% replacement of egg yolk lecithin with a combination of palmitoyl-lysolecithin and a free fatty acid (palmitate, stearate, oleate, linoleate, or arachidonate), followed by time-sequentially monitoring of vesicles and cholesterol crystals using spectrophotometer and video-enhanced differential contrast microscopy. Replacement with hydrophilic fatty acids (linoleate and arachidonate) reduced vesicle formation and promoted cholesterol crystallization, whereas an enhanced cholesterol-holding capacity was evident after replacement with hydrophobic fatty acids (palmitate and stearate). These results indicate that the effect of phospholipase A2 on bile lithogenecity is modulated by the fatty acid species in bile phospholipids, and therefore, that the role of phospholipase A2 in cholesterol gallstone formation is dependent, in part, on biliary phospholipid species selection at the site of hepatic excretion. [ABSTRACT FROM AUTHOR]
- Published
- 2000
- Full Text
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17. Bile salt hydrophobicity modulates subselection of biliary lecithin species in rats depleted of bile salt pool.
- Author
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Miyake, Hiroaki, Tazuma, Susumu, Kajiyama, Goro, Miyake, H, Tazuma, S, and Kajiyama, G
- Abstract
Although bile salts play an important role in the secretion of biliary lipid, little is known about the relationship between bile salt hydrophobicity and the selection of lecithin species to be secreted into bile. We therefore investigated whether bile salts modulate the selection of biliary lecithin subspecies. Rats that were depleted of the bile salt pool were infused with taurocholate (50, 100, 200, and 400 nmol/min/100 g body weight), taurochenodeoxycholate (25, 50, 100, and 200 nmol/min/100 g body weight), tauroursodeoxycholate (100, 200, 400, and 800 nmol/min/100 g body weight), or taurobetamuricholate (100, 200, 400, and 800 nmol/min/100 g body weight). Bile was collected to analyze bile flow, bile acid output, cholesterol levels, and lecithin levels. The hydrophobic-hydrophilic balance of the bile salts and biliary lecithin species was assessed by determining the retention times during reverse-phase high-performance liquid chromatography. Biliary lecithin secretion rates correlated with the hydrophobicity index of the biliary bile salts administered. Thus, biliary lecithin hydrophobicity increased with increasing bile salt hydrophobicity, whereas the molar cholesterol-lecithin ratio in the bile decreased. In conclusion, bile salt hydrophobicity regulates the selection of biliary lecithin subspecies during biliary secretion and thereby modulates, at least in part, bile cholesterol metastability. Thus, bile salt hydrophobicity accounts for the physicochemical conditions determining bile lipid metastability. [ABSTRACT FROM AUTHOR]
- Published
- 1998
- Full Text
- View/download PDF
18. Extracellular and intracellular regulation of biliary lecithin hydrophobicity.
- Author
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Miura, Hiroyuki, Tazuma, Susumu, Yamashita, Gunji, Kajiyama, Goro, Miura, H, Tazuma, S, Yamashita, G, and Kajiyama, G
- Abstract
Bromosulfophthalein and papaverine have been demonstrated to inhibit biliary lipid secretion without affecting secretion of bile salts in normal rats, so-called uncoupling. Bromosulfophthalein inhibits the capacity of intracanalicular bile salt micelles to induce biliary lipid secretion, and papaverine inhibits vesicular transport within the hepatocyte. We compared the effects of bromosulfophthalein and papaverine on biliary lipid secretion in normal Sprague-Dawley rats and Eizai hyperbilirubinuria rats. The fatty acyl chain saturation in biliary lecithin increased during bromosulfophthalein infusion and decreased during papaverine infusion in Sprague-Dawley rats. Bromosulfophthalein had no effect on biliary lipid secretion in Eizai rats, while papaverine induced uncoupling. The degree of fatty acyl chain saturation in biliary lecithin was unchanged during bromosulfophthalein infusion, but decreased with papaverine in Eizai rats. We deduce that selection of biliary lecithin species occurs at various points in the lipid transport pathway at intracellular and intracanalicular sites. [ABSTRACT FROM AUTHOR]
- Published
- 1998
- Full Text
- View/download PDF
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