1. A Survey of Regenerating Capacity in Patchy Liver and in Nodular Liver—with Special Reference to DNA Synthesis Estimated by BrdU Labeling Index
- Author
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Seiichi Takagi, Kunihiko Shindo, Kiyoshi Kubo, Yoshihiko Ito, Akio Shimizu, T. Mizuno, Atsushi Kiyohashi, Setsuo Tamai, Hiroshi Hoshino, Shiro Unayama, Yuko Oikawa, Yukifusa Kuni, Kazuto Iimori, and Kazuo Tarao
- Subjects
Pathology ,medicine.medical_specialty ,Brdu labeling ,medicine.diagnostic_test ,DNA synthesis ,Liver biopsy ,Significant difference ,Biopsy ,Gastroenterology ,medicine ,Immunohistochemistry ,Radiology, Nuclear Medicine and imaging ,Biology - Abstract
Laparoscopically, patchy liver and nodular liver were considered as features of regeneration of liver cells. In this study, the regenerating capacity of liver cells obtained by a liver biopsy from laparoscopically identified patchy liver and nodular liver was estimated by Bromodeoxvuridine (BrdU)-anti-BrdU method. BrdU labeling indices (L. I.), of liver cells in biopsy specimens from 6 normal livers, 12 patchy livers, and from 15 nodular livers were examined using an in-vitro labeling technique. Liver biopsy specimens obtained by a Tru-cut needle were immediately incubated for 45 min. in 0.1% BrdU solution in RPMI 1640 at 37°C under a pressure of 3 atmospheres in a mixture of 95% O2 and 5% CO2. Immunohistochemical detection of BrdU was performed by the Avidin-Biotin-Peroxidase Complex (ABC) method. The mean BrdU L. I. (±SE) of normal liver, patchy liver, and of nodular liver was 0.25±0.09%, 1.4±0.2%, and 1.7±0.4% respectively. Among the nodular liver, flat shaped ones showed a low level (0.5±0.1%), in contrast to the high level (2.4±0.7%) in the semispherical nodular liver. The BrdU L. I. of both the patchy liver and the nodular liver was significantly higher than that in the normal liver (p
- Published
- 1989
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