1. Upregulated TCRζ Enhances Interleukin-2 Production in T-Cells from Patients with CML.
- Author
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Zha, Xianfeng, Chen, Shaohua, Yang, Lijian, Shi, Li, Li, Bo, Wu, Xiuli, Lu, Yuhong, and Li, Yangqiu
- Subjects
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CHRONIC myeloid leukemia , *T cell receptors , *CELLULAR signal transduction , *INTERLEUKIN-2 , *GENE expression , *ENZYME-linked immunosorbent assay , *CELL-mediated cytotoxicity , *IMMUNOGLOBULINS - Abstract
T-cell immunodeficiency is a common feature in patients with chronic myeloid leukemia (CML), and deficiency in CD3 levels was detected in T cells from these patients, which may represent a characteristic that is related to a lower T cell activation. In this study, we explored the possibility that forced TCRζ gene expression may upreg-u-late T cell receptor (TCR) signaling activation and reverse interleukin-2 (IL-2) production in T cells from patients with CML. A recombinant eukaryotic vector expressing TCRζ was transfected into T cells by nucleofection. Phosphorylated TCRζ, phosphorylated NF-κB, and the IL-2 level in TCRζ-transfected CD3+T cells that were activated with anti-CD3 and anti-CD28 antibodies were measured by Western blot and enzyme-linked immunosorbent assay (ELISA). Significantly increased TCRζ levels were found in TCRζ-transfected CD3+T cells. After CD3 and CD28 antibody stimulation, a significantly higher phosphorylated TCRζ chain level was demonstrated, and an increased IL-2 production in TCRζ-upregulated T cells was associated with the increased expression of the phosphorylated NF-κB. In conclusion, TCRζ gene transfection could restore TCRζ chain deficiency and enhance IL-2 production in T cells from patients with CML. It is possible that TCRζ chain reconstitution in leukemia-specific, clonally expanded T cells will effectively increase their activation of antileukemia cytotoxicity. One chain of the T cell receptor TCRζ was overexpressed in CD3+T cells and following activation of those cells by antibody, the gene product was observed to be phosphorylated. The Ab-stimulated T cells produced more IL-2 and also had more activated NF-κB. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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