1. Isolation of the Carbon Catabolite Repressor ( CREA ) Gene from the Plant-pathogenic Fungus Cochliobolus carbonum.
- Author
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Tonukari, Nyerhovwo J., Scott-Craig, John S., and Walton, Jonathan D.
- Subjects
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GENETIC repressors , *PHYTOPATHOGENIC fungi - Abstract
The CREA gene has been implicated in glucose repression in several fungi. The product of this gene, CreA, binds to the promoter region of several enzymes and down-regulates gene expression. An ortholog of CREA was isolated and characterized from the maize pathogenic fungus, Cochliobolus carbonum . The deduced amino acid sequence of the C. carbonum CREA gene is very similar to the CreA proteins of Aspergillus niger , Gibberella fujikuroi , Sclerotinia sclerotiorum and Trichoderma reesei , as well as the Mig1 protein of Saccharomyces cerevisiae . And like the other fungal proteins, C. carbonum CreA has two zinc finger regions and a nuclear localization signal. Putative CreA binding sequences were also identified in the 5′ region of three C. carbonum cell wall degrading enzyme genes suggesting that the protein may play a role in the regulatory process that controls these enzymes expression. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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