1. Study of aflatoxicosis reduction: effect ofAlchornea cordifoliaon biomarkers in an aflatoxin B1 exposed rats
- Author
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Sébastien Djédjé Dano, Béatrice Tigori Sangaré, Pierre Manda, Jean-Baptiste Aholia Adépo, Jean Verdier N’gbe, and Aïssata Diakite
- Subjects
Male ,Aflatoxin ,Aflatoxin B1 ,Alchornea cordifolia ,Carcinogenesis ,Health, Toxicology and Mutagenesis ,macromolecular substances ,010501 environmental sciences ,Pharmacology ,Toxicology ,medicine.disease_cause ,01 natural sciences ,Antioxidants ,03 medical and health sciences ,Liver disease ,0302 clinical medicine ,Toxicity Tests, Acute ,medicine ,Animals ,heterocyclic compounds ,Rats, Wistar ,0105 earth and related environmental sciences ,Chemical Health and Safety ,Dose-Response Relationship, Drug ,biology ,Plant Extracts ,Lysine ,Euphorbiaceae ,technology, industry, and agriculture ,Public Health, Environmental and Occupational Health ,food and beverages ,Cancer ,General Medicine ,medicine.disease ,biology.organism_classification ,biological factors ,Aflatoxin B1-lysine adduct ,Plant Leaves ,Toxicity ,Aflatoxin M1 ,Biomarkers ,030217 neurology & neurosurgery - Abstract
The toxicity of aflatoxins results in cancer and liver disease. Several natural substances such as plants exhibited their ability to inhibit the initiation of aflatoxin carcinogenesis. The aim of this study was to evaluate the effect of Alchornea cordifolia on biomarkers in an aflatoxin B1 (AFB1) exposed rats. The contents of polyphenols, flavonoids and the antioxidant activity of A. cordifolia ethanolic leaf extract (EELac) were assessed. Groups of rats were treated orally with a daily dose of a mixture of AFB1 at a dose of 150 μg/kg body weight and EELac (50, 100 and 300 mg/kg body weight) for 21 days. Biomarkers of AFB1, such as the AFB1-lysine adduct and aflatoxin M1 were assayed in blood and urine, respectively, using an HPLC system with a fluorescence detector. The contents of polyphenols and flavonoids were 6783.23 ± 272.76 μg EAG/g and 10.54 ± 3.15% of dry matter, respectively. EELac showed a good antioxidant activity (IC50 = 12.65 ± 0.13 μg/mL). The administration of the mixture (AFB1 + EELac) at different doses significantly reduced the level of AFB1-lysine adduct from 14.04 ± 2.1 to 4.13 ± 0.9 ng/mg albumin and that of Aflatoxin M1 (AFM1) from 456 ± 16 to 220 ± 24 ng/mL (p 0.05). The rate of reduction was 70.58% for AFB1-lysine adduct and 51.75% for AFM1. A. cordifolia could be used in the prevention of toxicity induced by AFB1 on account of its high content in phenolic compounds.
- Published
- 2018