1. Identification of a Novel Glutathione Conjugate of Diclofenac by LTQOrbitrap
- Author
-
Brian K. Albrecht, Yohannes Teffera, Zhiyang Zhao, Adria Colletti, and Daniel Waldon
- Subjects
Male ,inorganic chemicals ,Diclofenac ,Decarboxylation ,Clinical Biochemistry ,Pharmaceutical Science ,High resolution ,Mass Spectrometry ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,medicine ,Animals ,Humans ,Pharmacology (medical) ,Benzoic acid ,Chromatography ,organic chemicals ,Anti-Inflammatory Agents, Non-Steroidal ,Biochemistry (medical) ,Glutathione ,Rats ,stomatognathic diseases ,Human hepatocyte ,chemistry ,Biochemistry ,Hepatocytes ,Microsomes, Liver ,Chromatography, Liquid ,medicine.drug ,Conjugate - Abstract
High resolution accurate MS with an LTQ-Orbitrap identified two novel metabolites of diclofenac in rat bile and rat and human hepatocyte incubations: a benzyl-S-glutathione conjugate and 2-(2,6-dichlorophenylamino) benzoic acid. A mechanism for the bioactivation of diclofenac involving decarboxylation is proposed.
- Published
- 2008