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Commonly known as “sea spiders”, pycnogonids are a group of exclusively marine arthropods. In Mexico, 62 species of Pycnogonida have been reported from almost all the coastal Mexican states. This data is the result mostly of investigations carried out by foreigners and recent studies of sporadic collections focused on meiobenthos fauna. The aim of this paper is to disclose the samples of pycnogonids contained in the National Collection of Mites (CNAC), which was part of PhD. Anita Hoffmann’s collection and which was collected from two different locations in the Mexican Pacific by PhD. Federico Bonet in the 1940s. Three species were identified from the total of 20 specimens: Achelia sawayai (Montagu, 1808), Anoplodactylus californicus Hall, 1912, and Endeis cf. spinosa Marcus, 1940. For the first species, a record is added to the already known wide distribution. E. cf. spinosa was recorded for the first time in the Mexican Pacific, and for A. sawayai, records of its distribution in the country are expanded. Furthermore, the importance of biological collections as material sites for the recognition of biodiversity is highlighted. [ABSTRACT FROM AUTHOR]