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Real-time monitoring of the variation of redox is significant for the dynamic reflecting of the degree of oxidative stress in biological systems. Most of the fluorescent probes can respond to monotonically increased redox, but fail to respond to the fluctuation. Fortunately, the emergence of reversible probes overcame the above issue. Therefore, a review of the design strategies of reversible fluorescence probes for redox would help to develop advanced tools for complicated bio-microenvironments. In this review, we focused on the research progress of reversible fluorescent probes for redox in terms of response sites, including chalcogen atoms (S, Se, Te), quinones, C C bonds, and other types. In each category, we summarized the design strategies and response mechanisms: prone to fluctuated redox of chalcogen atoms; the transformation between hydroquinone and quinone; nucleophilic reaction site (C C bonds) for reactive sulfur species. Moreover, real-time monitoring of redox in cellular, zebrafish and mice was discussed. Finally, future opportunities and challenges in the clinical translation of reversible fluorescent probes, for example, low toxicity, long excitation and emission wavelengths, short response times, and high sensitivity were discussed. We hope this review may promote the development of more robust reversible fluorescent probes to further understand redox-related pathological events. The design strategies and response mechanisms of reversible fluorescence probes for redox were summarized according to different response sites, including chalcogen atoms (S, Se, Te), quinones, C C bonds, and so on, which would be helpful to promote the development of more robust reversible fluorescent probes for redox to gain a detailed understanding of oxidative stress-related pathological events. [Display omitted] • We focused on the research progress of reversible fluorescent probes for redox in terms of response sites. • We summarized the design strategies and response mechanisms, and biological application. • Future opportunities and challenges in the clinical translation of reversible fluorescent probes were discussed. [ABSTRACT FROM AUTHOR]

Two functionalized rhodamine based probes have been rational designed and synthesized. The probes have sufficiently high selectivity and sensitivity for the detection of Fe 3+ ion both by fluorescence and ultraviolet spectroscopy with strong ability of anti-interference performance. The detection process has been shown to be reversible and involves the fluoran ring-opening upon complexation with Fe 3+ . The obvious fluorescence image obtained from confocal laser scanning microscopy of the detection of Fe 3+ in living L929 cells demonstrate that the probes could contribute to significant breakthroughs in understanding the critically important functions of Fe 3+ in related cells and biological organs. [ABSTRACT FROM AUTHOR]