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The research takes low C/N ratio and high ammonia nitrogen sewage as the treatment object, connects the SBBR reactor and UASB reactor after stable operation in series, and studies the PN-ANAMMOX coupling mechanism. The research analyze the effect of the influencing factors of the nitrification process on the subsequent anaerobic ammonia oxidation stage. The two stages adapt to each other that constantly debug and run. The research investigate its denitrification effect, analyze the biological denitrification mechanism and determine the optimal working conditions.