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Conservation planning processes and wetland management require spatial estimations of aquatic habitats to support the maintenance of aquatic biodiversity. However, physical access to several wetlands and freshwater habitats can be restricted due to difficult topography and technological limitations associated with ground-based observations. In addition to these constraints, the distribution of some aquatic primary producers in freshwater habitats and floodplains that are difficult to reach further complicates large-scale assessment of aquatic habitats using traditional field-based techniques. The main objective of this study is to predict the spatial distribution of hot spots of primary producers (aquatic plant biomass) and assess large-scale inundation patterns in a large floodplain wetland (Flinders catchment) in the wet-dry tropics of Australia. To this end, remote sensing biophysical indicators (vegetation and inundation) were integrated with flood water depth in a classification tree model. Results indicate that in terms of floodplain total inundation, the Flinders wetland hydrology is rather restricted immediately after the summer wet season, the period when most primary production happens. While this can be attributed to the fact that much of the observed annual variability (93%) in rainfall and surface runoff (95%) occur during the wet season, post flood recession patterns are indicators that underpin the somewhat limited alimentation of the Flinders floodplain during this period. As observed in this study, post flood inundation extents in the summers of 2009 and 2019 declined by approximately 89% and 87% within fourteen and ten days, respectively. Despite having a significantly higher magnitude than the 2009 summer flood event, the 2019 extreme 'big wet' period did not translate to higher floodplain productivity (aquatic plant biomass and surface water distribution) immediately after summer as was the case in 2009. Furthermore, the predicted extents of aquatic plant biomass and total floodplain inundation in the downstream Flinders show substantial temporal variation and suggest the floodplain wetland hydrology is largely driven by inter-annual changes in annual rainfall. The extents of these hot spots of biomass accumulation were found to be considerably associated with total floodplain inundation extent (r = 0.94), rainfall (r = 0.81), and discharge (r = 0.68). Furthermore, advance statistical analyses show that downstream discharge and rainfall over the Flinders are significantly correlated (r = 0.72). In addition to this, observed amplitudes of discharge and rainfall in extreme wet and dry years coincided with floodplain inundation patterns and distribution of hot spots of primary producers. While these relationships emphasize the importance of flow in the nourishment of the downstream catchment, they further corroborate the composite influence of local rainfall and discharge on total floodplain inundation and hot spots of primary producers. [ABSTRACT FROM AUTHOR]