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Abstract: A specially tailored electrochemical biosensor for ultrasensitive detection of BCR/ABL fusion gene was developed with high selectivity, acceptable rapidity and excellent extensibility. Taking advantage of circular strand replacement polymerization, cascade hybridization reaction as well as quantum dot based signal amplification, the proposed biosensor showed high sensitivity with the detection limit down to 2fM. This strategy offers a great promise to early diagnosis and prognosis of chronic myeloid leukemia. [Copyright &y& Elsevier]

Abstract: MicroRNA-16 (miR-16) frequently deleted or downregulated in patients with B cell chronic lymphocytic leukemia and thus can be used as promising biomarkers in B-CLL diagnosis and prognosis. Herein, an ultrasensitive miR-16 detection was carried out by combining rolling circle amplification (RCA), quantum dots tagging and anodic stripping voltammetric detection (ASV). The method could specifically quantify miR-16 over a 6-decade dynamic range and reach an ultra-low detection limit of 0.32 aM. [Copyright &y& Elsevier]

Abstract: The hollow spherical CdSe QD assemblies were synthesized via a sonochemical approach that utilizes β-cyclodextrin as a template reagent in aqueous solution. The hollow nanospheres have an average diameter of 70nm and are found to consist of an assembly of monodispersed 5nm sized CdSe quantum dots. Following an electrochemical reaction with persulfate ions, strong electrogenerated chemiluminescence (ECL) was observed from the CdSe nanoassemblies suspended in an aqueous solution of pH⩽7.95. The study indicates that the morphology of the 70nm nanoassembly plays an important role in generating the stable ECL since individually dispersed quantum dots did not exhibit any significant ECL. The unique ECL intensity and stability of the synthesized spherical nanoassemblies could allow for potential sensor applications of CdSe quantum dots in water. [Copyright &y& Elsevier]

Abstract: A novel quantum dots (QDs) ECL biosensor for the detection of lysozyme was developed. Lysozyme was first incubated with probes immobilized at Au electrode in order to form the aptamer-lysozyme bioaffinity complexes. And the free probes were hybridized with the 5′-biotin modified cDNA oligonucleotides to form double-stranded DNA (ds-DNA) oligonucleotides. Avidin-QDs were bound to these hybridized cDNA through the biotin-avidin-system. The ECL signal of the biosensor was responsive to the amount of QDs bonded to the cDNA oligonucleotides, which was indirectly inverse proportional to the combined target protein. [Copyright &y& Elsevier]