1. Investigation on the effect of static magnetic field up to 30 mT on viability percent, proliferation rate and IC50 of HeLa and fibroblast cells
- Author
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Mohammad Javad Khodayar, Amir Jalali, Fatemeh Javani Jouni, Jaber Zafari, and Parviz Abdolmaleki
- Subjects
Cell type ,Cell growth ,Cell Survival ,Cell ,Biophysics ,Medicine (miscellaneous) ,General Medicine ,Biology ,Fibroblasts ,biology.organism_classification ,Molecular biology ,Cell biology ,HeLa ,Inhibitory Concentration 50 ,medicine.anatomical_structure ,Magnetic Fields ,Cell culture ,medicine ,Humans ,MTT assay ,Viability assay ,Fibroblast ,Cell Proliferation ,HeLa Cells - Abstract
We have investigated the effects of static magnetic field (SMF) on the viability of the human cervical cancer (HeLa) cell line and fibroblast cells. The cells were cultured in DMEM medium and treated several times (24, 48,72 and 96 h) and at several intensities (5, 10, 20 and 30 mT) of magnetic field (MF). The cytotoxicity and cell viability percent in treated cells were performed using MTT assay by evaluating mitochondrial dehydrogenase activity. The MF ability on inducing cell death or inhibiting biochemical function was reported as cell death percent. The results showed that the increase of MF intensity and the time that cells were exposed to this treatment increased sharply cell death percent and proliferation rate in HeLa cell compare to fibroblast cells. Our data suggest that SMF biological effects on cell death were different in our selected targets. Cell type and time of exposure have been therefore found to be significant factors. These findings could be used to improve new effective method using SMF in conjunction with the common therapeutic approaches.
- Published
- 2015