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Due to the nature of hospital operations, there are some difficulties in performing cost analysis studies. This study aims to provide useful information to analysts and related managers by revealing what kind of difficulties are encountered while performing cost analysis applications in hospital operations. For this purpose, research data have been obtained by using a questionnaire prepared for senior executives who work for private hospitals, the Ministry of Health hospitals, university hospitals, and city hospitals operating in Turkey. The data obtained from the research have been analyzed by using the SPSS 22.0 package program. As a result of the analysis performed, it has been reached findings that related descriptive statistics and hypothesis tests. According to the findings, a cost analysis was not performed in 48'1% of MOH hospitals, a cost analysis was performed in the medical/accounting department in 49% of private hospitals and 50% of city hospitals and the finance department in 46.8% of university hospitals. The most common difficulties are as follows: Healthcare services are highly complex and variable, the complexity of expense types, locations, and centers, have a sales price set by the government regardless of the cost of the service produced in the hospital; and The price of the service produced is not determined by the cost accounts but Health Application Communiqué (HAC). Accordingly, hospital managers do not give the necessary importance to cost analysis and the difficulties they face are thought to be due to their specific characteristics of health services and cost elements. [ABSTRACT FROM AUTHOR]