1. The S-phase-induced lncRNA SUNO1 promotes cell proliferation by controlling YAP1/Hippo signaling pathway
- Author
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Maria Polycarpou-Schwarz, Lisa M. Miller Jenkins, Arturo V. Orjalo, Yuelin J. Zhu, Sarath Chandra Janga, You Jin Song, Ashish Lal, Qinyu Hao, Anindya Bagchi, Arindam Chakraborty, Supriya G. Prasanth, Kannanganattu V. Prasanth, Deepak Singh, Sven Diederichs, Mohammad Kamran, Xinying Zong, Rosaline Y.C. Hsu, Seyedsasan Hashemikhabir, Xiaoling Li, Yo Chuen Lin, Yoon Jung Kim, Hans E. Johansson, Qinyu Sun, Vidisha Tripathi, Tae Hoon Kim, Ritu Chaudhary, and Branden S. Moriarity
- Subjects
0301 basic medicine ,QH301-705.5 ,Science ,Biology ,HCT116 ,General Biochemistry, Genetics and Molecular Biology ,S Phase ,DEAD-box RNA Helicases ,Transcriptome ,03 medical and health sciences ,lncRNA ,0302 clinical medicine ,U2OS ,Transcription (biology) ,None ,Gene expression ,Humans ,Biology (General) ,Cell Proliferation ,YAP1 ,Hippo signaling pathway ,General Immunology and Microbiology ,Cell growth ,General Neuroscience ,General Medicine ,Cell cycle ,Chromosomes and Gene Expression ,HCT116 Cells ,Up-Regulation ,Cell biology ,Chromatin ,Cytoskeletal Proteins ,030104 developmental biology ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,WTIP ,Medicine ,cell cycle ,RNA, Long Noncoding ,human cancer cell lines ,Co-Repressor Proteins ,Research Article ,HeLa Cells ,Signal Transduction - Abstract
Cell cycle is a cellular process that is subject to stringent control. In contrast to the wealth of knowledge of proteins controlling the cell cycle, very little is known about the molecular role of lncRNAs (long noncoding RNAs) in cell-cycle progression. By performing genome-wide transcriptome analyses in cell-cycle-synchronized cells, we observed cell-cycle phase-specific induction of >2000 lncRNAs. Further, we demonstrate that an S-phase-upregulated lncRNA, SUNO1, facilitates cell-cycle progression by promoting YAP1-mediated gene expression. SUNO1 facilitates the cell-cycle-specific transcription of WTIP, a positive regulator of YAP1, by promoting the co-activator, DDX5-mediated stabilization of RNA polymerase II on chromatin. Finally, elevated SUNO1 levels are associated with poor cancer prognosis and tumorigenicity, implying its pro-survival role. Thus, we demonstrate the role of a S-phase up-regulated lncRNA in cell-cycle progression via modulating the expression of genes controlling cell proliferation.
- Published
- 2020
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