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9 results on '"Castillo, Pablo"'

6. Oxytocin improves behavioral and electrophysiological deficits in a novel Shank3-deficient rat

Author

Harony-Nicolas, Hala, primary, Kay, Maya, additional, Hoffmann, Johann du, additional, Klein, Matthew E, additional, Bozdagi-Gunal, Ozlem, additional, Riad, Mohammed, additional, Daskalakis, Nikolaos P, additional, Sonar, Sankalp, additional, Castillo, Pablo E, additional, Hof, Patrick R, additional, Shapiro, Matthew L, additional, Baxter, Mark G, additional, Wagner, Shlomo, additional, and Buxbaum, Joseph D, additional

Published
2017
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9. CB 1 -receptor-mediated inhibitory LTD triggers presynaptic remodeling via protein synthesis and ubiquitination.

Author

Monday HR, Bourdenx M, Jordan BA, and Castillo PE

Subjects
Actin-Related Protein 2-3 Complex metabolism, Animals, Female, Hippocampus chemistry, Hippocampus cytology, Hippocampus metabolism, Male, Rats, Rats, Sprague-Dawley, Wiskott-Aldrich Syndrome Protein Family metabolism, Long-Term Synaptic Depression physiology, Protein Biosynthesis physiology, Receptor, Cannabinoid, CB1 metabolism, Ubiquitination physiology
Abstract

Long-lasting forms of postsynaptic plasticity commonly involve protein synthesis-dependent structural changes of dendritic spines. However, the relationship between protein synthesis and presynaptic structural plasticity remains unclear. Here, we investigated structural changes in cannabinoid-receptor 1 (CB 1 )-mediated long-term depression of inhibitory transmission (iLTD), a form of presynaptic plasticity that involves a protein-synthesis-dependent long-lasting reduction in GABA release. We found that CB 1 -iLTD in acute rat hippocampal slices was associated with protein synthesis-dependent presynaptic structural changes. Using proteomics, we determined that CB 1 activation in hippocampal neurons resulted in increased ribosomal proteins and initiation factors, but decreased levels of proteins involved in regulation of the actin cytoskeleton, such as ARPC2 and WASF1/WAVE1, and presynaptic release. Moreover, while CB 1 -iLTD increased ubiquitin/proteasome activity, ubiquitination but not proteasomal degradation was critical for structural and functional presynaptic CB 1 -iLTD. Thus, CB 1 -iLTD relies on both protein synthesis and ubiquitination to elicit structural changes that underlie long-term reduction of GABA release., Competing Interests: HM, MB, BJ, PC No competing interests declared, (© 2020, Monday et al.)

Published
2020
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