1. G-quadruplex RNA structure as a signal for neurite mRNA targeting
- Author
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Hervé Moine, Eric Flatter, Ricardos Tabet, Murugan Subramanian, Florence Rage, Jean-Louis Mandel, Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Chaire Génétique Humaine, and Collège de France (CdF (institution))
- Subjects
Neurite ,Molecular Sequence Data ,RNA transport ,Biology ,G-quadruplex ,Biochemistry ,RNA Transport ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Genetics ,Neurites ,Animals ,Humans ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,heterocyclic compounds ,Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinase Type 2/genetics *G-Quadruplexes Humans Intracellular Signaling Peptides and Proteins/genetics Membrane Proteins/genetics Mice Mice ,RNA, Messenger ,Nucleic acid structure ,Molecular Biology ,030304 developmental biology ,AU-rich element ,0303 health sciences ,Messenger RNA ,Base Sequence ,Scientific Reports ,Intracellular Signaling Peptides and Proteins ,RNA ,Membrane Proteins ,Cell biology ,G-Quadruplexes ,Mice, Inbred C57BL ,Metabotropic receptor ,nervous system ,Messenger/*chemistry/*metabolism ,Nucleic Acid Conformation ,Inbred C57BL Molecular Sequence Data Neurites/*metabolism *Nucleic Acid Conformation RNA Transport RNA ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,Disks Large Homolog 4 Protein ,030217 neurology & neurosurgery - Abstract
International audience; Targeting of messenger RNAs (mRNAs) in neuron processes relies on cis-acting regulatory elements, the nature of which is poorly understood. Here, we report that approximately 30% of the best-known dendritic mRNAs contain a guanine (G)-quadruplex consensus in their 3'-untranslated region. Among these mRNAs, we show by using RNA structure probing that a G-quadruplex is present in the mRNAs of two key postsynaptic proteins: PSD-95 and CaMKIIa. The G-quadruplex structure is necessary and sufficient for the potent and fast localization of mRNAs in cortical neurites and this occurs in a metabotropic glutamate receptor-responsive manner. Thus, G-quadruplex seems to be a common neurite localization signal.
- Published
- 2011
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