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Your search keyword '"Direct sequencing"' showing total 5 results
Start Over Descriptor "Direct sequencing" Journal emerging infectious diseases
5 results on '"Direct sequencing"'

1. Full Genome of Influenza A (H7N9) Virus Derived by Direct Sequencing without Culture

Author

Xianwen Ren, Fan Yang, Yongfeng Hu, Ting Zhang, Liguo Liu, Jie Dong, Lilian Sun, Yafang Zhu, Yan Xiao, Li Li, Jian Yang, Jianwei Wang, and Qi Jin

Subjects
H7N9, influenza A virus, deep sequencing, culture-free, avian-origin, direct sequencing, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

An epidemic caused by influenza A (H7N9) virus was recently reported in China. Deep sequencing revealed the full genome of the virus obtained directly from a patient’s sputum without virus culture. The full genome showed substantial sequence heterogeneity and large differences compared with that from embryonated chicken eggs.

Published
2013
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2. Full genome of influenza A (H7N9) virus derived by direct sequencing without culture.

Author

Ren, Xianwen, Yang, Fan, Hu, Yongfeng, Zhang, Ting, Liu, Liguo, Dong, Jie, Sun, Lilian, Zhu, Yafang, Xiao, Yan, Li, Li, Yang, Jian, Wang, Jianwei, and Jin, Qi

Abstract

An epidemic caused by influenza A (H7N9) virus was recently reported in China. Deep sequencing revealed the full genome of the virus obtained directly from a patient's sputum without virus culture. The full genome showed substantial sequence heterogeneity and large differences compared with that from embryonated chicken eggs. [ABSTRACT FROM AUTHOR]

Published
2013
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3. Rapid Genome Sequencing of RNA Viruses

Author

Tetsuya Mizutani, Daiji Endoh, Michiko Okamoto, Kazuya Shirato, Hiroyuki Shimizu, Minetaro Arita, Shuetsu Fukushi, Masayuki Saijo, Chang Kweng Lim, Mikako Ito, Reiko Nerome, Tomohiko Takasaki, Koji Ishii, Tetsuro Suzuki, Ichiro Kurane, Shigeru Morikawa, and Hidekazu Nishimura

Subjects
Emerging infectious virus, SARS-CoV, West Nile virus, rapid determination system, whole genome amplification, direct sequencing, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.

Published
2007
Full Text
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4. Full Genome of Influenza A (H7N9) Virus Derived by Direct Sequencing without Culture

Author

Jian Yang, Jie Dong, Li Li, Yongfeng Hu, Qi Jin, Yan Xiao, Jianwei Wang, Lilian Sun, Xianwen Ren, Fan Yang, Ting Zhang, Yafang Zhu, and Liguo Liu

Subjects
Microbiology (medical), China, Epidemiology, viruses, Molecular Sequence Data, lcsh:Medicine, culture-free, Hemagglutinin Glycoproteins, Influenza Virus, Chick Embryo, Genome, Viral, Biology, Influenza A Virus, H7N9 Subtype, medicine.disease_cause, Genome, avian-origin, Virus, Deep sequencing, lcsh:Infectious and parasitic diseases, H7N9, deep sequencing, Phylogenetics, Influenza, Human, Influenza A virus, medicine, Animals, Humans, influenza A virus, lcsh:RC109-216, Phylogeny, Genetics, Viral culture, lcsh:R, Sputum, Dispatch, Embryonated, High-Throughput Nucleotide Sequencing, Sequence Analysis, DNA, direct sequencing, Middle Aged, Virology, Infectious Diseases, Female, medicine.symptom, influenza
Abstract

An epidemic caused by influenza A (H7N9) virus was recently reported in China. Deep sequencing revealed the full genome of the virus obtained directly from a patient’s sputum without virus culture. The full genome showed substantial sequence heterogeneity and large differences compared with that from embryonated chicken eggs.

Published
2013

5. Rapid Genome Sequencing of RNA Viruses

Author

Daiji Endoh, Mikako Ito, Tetsuya Mizutani, Hiroyuki Shimizu, Tetsuro Suzuki, Koji Ishii, Shuetsu Fukushi, Tomohiko Takasaki, Kazuya Shirato, Masayuki Saijo, Ichiro Kurane, Michiko Okamoto, Kouji Sakai, Chang Kweng Lim, Reiko Nerome, Shigeru Morikawa, Minetaro Arita, and Hidekazu Nishimura

Subjects
Microbiology (medical), DNA, Complementary, Time Factors, Epidemiology, viruses, lcsh:Medicine, Genomics, Genome, Viral, Biology, Genome, Virus, DNA sequencing, lcsh:Infectious and parasitic diseases, Plasmid, Emerging infectious virus, RNA Viruses, lcsh:RC109-216, Genetics, Whole Genome Amplification, Base Sequence, whole genome amplification, lcsh:R, Dispatch, RNA, SARS-CoV, direct sequencing, Infectious Diseases, Subcloning, rapid determination system, RNA, Viral, Nucleic Acid Amplification Techniques, West Nile virus
Abstract

We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.

Published
2007

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