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Start Over Descriptor "NAT" Journal emerging infectious diseases
9 results on '"NAT"'

1. Signs or Symptoms of Acute HIV Infection in a Cohort Undergoing Community-Based Screening.

Author

Hoenigl, Martin, Green, Nella, Camacho, Martha, Gianella, Sara, Mehta, Sanjay R, Smith, Davey M, and Little, Susan J

Subjects
Humans, HIV-1, HIV Infections, Acute Disease, Mass Screening, CD4 Lymphocyte Count, Viral Load, Cohort Studies, Homosexuality, Male, Adolescent, Adult, Aged, Middle Aged, California, Female, Male, Young Adult, HIV/AIDS and other retroviruses, MSM, NAT, acute HIV infection, acute retroviral syndrome, men who have sex with men, nucleic acid amplification, screening, seroconversion, signs, symptoms, testing, typical acute retroviral syndrome, viruses, HIV/AIDS, Infectious Diseases, Mental Health, Clinical Research, 4.2 Evaluation of markers and technologies, Infection, Acute Disease Adolescent Adult Aged CD4 Lymphocyte Count California/epidemiology Cohort Studies Female HIV Infections/*diagnosis/epidemiology/virology HIV-1/classification/genetics Homosexuality, Male Humans Male Mass Screening Middle Aged Viral Load Young Adult HIV/AIDS and other retroviruses Msm Nat acute HIV infection acute retroviral syndrome men who have sex with men nucleic acid amplification screening seroconversion signs symptoms testing typical acute retroviral syndrome viruses, Microbiology, Clinical Sciences, Medical Microbiology, Public Health and Health Services
Abstract

We analyzed signs and symptoms in 90 patients diagnosed with acute HIV infection in a community-based program that offered universal HIV-1 nucleic acid amplification testing. Forty-seven (52%) patients reported ongoing signs or symptoms at the time of testing. Another 25 (28%) reported signs or symptoms that had occurred during the 14 days before testing.

Published
2016

2. Signs or Symptoms of Acute HIV Infection in a Cohort Undergoing Community-Based Screening

Author

Martin Hoenigl, Nella Green, Martha Camacho, Sara Gianella, Sanjay R. Mehta, Davey M. Smith, and Susan J. Little

Subjects
acute HIV infection, HIV/AIDS and other retroviruses, men who have sex with men, MSM, seroconversion, NAT, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

We analyzed signs and symptoms in 90 patients diagnosed with acute HIV infection in a community-based program that offered universal HIV-1 nucleic acid amplification testing. Forty-seven (52%) patients reported ongoing signs or symptoms at the time of testing. Another 25 (28%) reported signs or symptoms that had occurred during the 14 days before testing.

Published
2016
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3. World Health Organization International Standard to Harmonize Assays for Detection of Hepatitis E Virus RNA

Author

Sally A. Baylis, Johannes Blümel, Saeko Mizusawa, Keiji Matsubayashi, Hidekatsu Sakata, Yoshiaki Okada, C. Micha Nübling, and Kay-Martin O. Hanschmann

Subjects
hepatitis E virus, HEV, NAT, standardization, RNA, hepatitis, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Nucleic acid amplification technique–based assays are a primary method for the detection of acute hepatitis E virus (HEV) infection, but assay sensitivity can vary widely. To improve interlaboratory results for the detection and quantification of HEV RNA, a candidate World Health Organization (WHO) International Standard (IS) strain was evaluated in a collaborative study involving 23 laboratories from 10 countries. The IS, code number 6329/10, was formulated by using a genotype 3a HEV strain from a blood donation, diluted in pooled human plasma and lyophilized. A Japanese national standard, representing a genotype 3b HEV strain, was prepared and evaluated in parallel. The potencies of the standards were determined by qualitative and quantitative assays. Assay variability was substantially reduced when HEV RNA concentrations were expressed relative to the IS. Thus, WHO has established 6329/10 as the IS for HEV RNA, with a unitage of 250,000 International Units per milliliter.

Published
2013
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4. Signs or Symptoms of Acute HIV Infection in a Cohort Undergoing Community-Based Screening

Author

Susan J. Little, Nella Green, Martha Camacho, Martin Hoenigl, Davey M. Smith, Sanjay Mehta, and Sara Gianella

Subjects
Male, 0301 basic medicine, Pediatric AIDS, Pediatrics, Epidemiology, men who have sex with men, lcsh:Medicine, HIV Infections, Acute Disease Adolescent Adult Aged CD4 Lymphocyte Count California/epidemiology Cohort Studies Female HIV Infections/*diagnosis/epidemiology/virology HIV-1/classification/genetics Homosexuality, Male Humans Male Mass Screening Middle Aged Viral Load Young Adult HIV/AIDS and other retroviruses Msm Nat acute HIV infection acute retroviral syndrome men who have sex with men nucleic acid amplification screening seroconversion signs symptoms testing typical acute retroviral syndrome viruses, California, Men who have sex with men, Cohort Studies, acute HIV infection, 0302 clinical medicine, Mass Screening, 030212 general & internal medicine, Screening cultures, Pediatric, Community based, Dispatch, Homosexuality, Middle Aged, Viral Load, Signs or Symptoms of Acute HIV Infection in a Cohort Undergoing Community-Based Screening, testing, nucleic acid amplification, 3. Good health, Mental Health, Infectious Diseases, Medical Microbiology, Acute Disease, Cohort, Public Health and Health Services, HIV/AIDS, Female, Infection, 4.2 Evaluation of markers and technologies, Adult, Microbiology (medical), Male Humans Male Mass Screening Middle Aged Viral Load Young Adult HIV/AIDS and other retroviruses Msm Nat acute HIV infection acute retroviral syndrome men who have sex with men nucleic acid amplification screening seroconversion signs symptoms testing typical acute retroviral syndrome viruses, medicine.medical_specialty, Adolescent, Clinical Sciences, acute retroviral syndrome, Signs and symptoms, Microbiology, lcsh:Infectious and parasitic diseases, Young Adult, 03 medical and health sciences, typical acute retroviral syndrome, Clinical Research, medicine, Humans, signs, viruses, lcsh:RC109-216, MSM, Homosexuality, Male, Seroconversion, HIV/AIDS and other retroviruses, seroconversion, Aged, Acute HIV infection, business.industry, screening, lcsh:R, CD4 Lymphocyte Count, Acute Retroviral Syndrome, Good Health and Well Being, 030104 developmental biology, HIV-1, Acute Disease Adolescent Adult Aged CD4 Lymphocyte Count California/epidemiology Cohort Studies Female HIV Infections/*diagnosis/epidemiology/virology HIV-1/classification/genetics Homosexuality, symptoms, business, NAT
Abstract

We analyzed signs and symptoms in 90 patients diagnosed with acute HIV infection in a community-based program that offered universal HIV-1 nucleic acid amplification testing. Forty-seven (52%) patients reported ongoing signs or symptoms at the time of testing. Another 25 (28%) reported signs or symptoms that had occurred during the 14 days before testing.

Published
2016
Full Text
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5. World Health Organization International Standard to Harmonize Assays for Detection of Hepatitis E Virus RNA

Author

Johannes Blümel, Keiji Matsubayashi, Hidekatsu Sakata, Kay-Martin Hanschmann, C. Micha Nübling, Saeko Mizusawa, Sally A. Baylis, and Yoshiaki Okada

Subjects
Microbiology (medical), Epidemiology, assays, genotype, International Cooperation, viruses, detection, lcsh:Medicine, hepatitis E virus, Biology, medicine.disease_cause, World Health Organization, Global Health, Sensitivity and Specificity, Virus, lcsh:Infectious and parasitic diseases, strains, WHO, Hepatitis E virus, Genotype, medicine, Humans, genotype 3, hepatitis, lcsh:RC109-216, subgenotype, Phylogeny, Hepatitis, standardization, Research, lcsh:R, RNA, virus diseases, Nucleic acid amplification technique, Assay sensitivity, Hepatitis E, medicine.disease, Virology, genotype 3a, Infectious Diseases, nucleic acid amplification technique, HEV, RNA, Viral, Biological Assay, NAT, international standard
Abstract

Nucleic acid amplification technique–based assays are a primary method for the detection of acute hepatitis E virus (HEV) infection, but assay sensitivity can vary widely. To improve interlaboratory results for the detection and quantification of HEV RNA, a candidate World Health Organization (WHO) International Standard (IS) strain was evaluated in a collaborative study involving 23 laboratories from 10 countries. The IS, code number 6329/10, was formulated by using a genotype 3a HEV strain from a blood donation, diluted in pooled human plasma and lyophilized. A Japanese national standard, representing a genotype 3b HEV strain, was prepared and evaluated in parallel. The potencies of the standards were determined by qualitative and quantitative assays. Assay variability was substantially reduced when HEV RNA concentrations were expressed relative to the IS. Thus, WHO has established 6329/10 as the IS for HEV RNA, with a unitage of 250,000 International Units per milliliter.

Published
2013

6. External Quality Assessment for Zika Virus Molecular Diagnostic Testing, Brazil

Author

Johannes Blümel and Sally A. Baylis

Subjects
Microbiology (medical), Letter, Epidemiology, 030231 tropical medicine, lcsh:Medicine, World Health Organization, lcsh:Infectious and parasitic diseases, Zika virus, 03 medical and health sciences, 0302 clinical medicine, armored RNA, External quality assessment, Medicine, Humans, lcsh:RC109-216, viruses, controls, NAAT, 030212 general & internal medicine, inactivation, Letters to the Editor, External Quality Assessment for Zika Virus Molecular Diagnostic Testing, Brazil, biology, Molecular Diagnostic Testing, business.industry, Zika Virus Infection, lcsh:R, Nucleic acid amplification technique, Zika Virus, Viral Load, biology.organism_classification, external quality assessment, Virology, Infectious Diseases, nucleic acid amplification technique, Molecular Diagnostic Techniques, business, NAT, international standard, Brazil
Published
2018

7. SARS molecular detection external quality assurance

Author

Matthias Niedrig, Hans Wilhelm Doerr, Wilina Lim, Christian Drosten, and Klaus Stöhr

Subjects
Microbiology (medical), Quality Control, Epidemiology, Biology, medicine.disease_cause, World health, WHO, Sars virus, medicine, Humans, Coronavirus, SARS, business.industry, Reverse Transcriptase Polymerase Chain Reaction, Dispatch, Reproducibility of Results, Virology, Quality assurance, Infectious Diseases, PCR, Multicenter study, Severe acute respiratory syndrome-related coronavirus, RNA, Viral, business, Laboratories, NAT
Abstract

Inactivated severe acute respiratory syndrome-associated coronavirus samples were used for an external quality assurance study within the World Health Organization SARS Reference and Verification Network and other reference institutions. Of 58 participants, 51 correctly detected virus in all samplesor =9,400 RNA copies per milliliter and none in negative samples. Commercial test kits significantly improved the outcome.

Published
2005

9. World Health Organization International Standard to harmonize assays for detection of hepatitis E virus RNA.

Author

Baylis SA, Blümel J, Mizusawa S, Matsubayashi K, Sakata H, Okada Y, Nübling CM, and Hanschmann KM

Subjects
Global Health, Hepatitis E blood, Hepatitis E virology, Hepatitis E virus classification, Hepatitis E virus isolation & purification, Humans, International Cooperation, Phylogeny, RNA, Viral blood, Sensitivity and Specificity, World Health Organization, Biological Assay standards, Hepatitis E diagnosis, Hepatitis E virus genetics, RNA, Viral genetics
Abstract

Nucleic acid amplification technique-based assays are a primary method for the detection of acute hepatitis E virus (HEV) infection, but assay sensitivity can vary widely. To improve interlaboratory results for the detection and quantification of HEV RNA, a candidate World Health Organization (WHO) International Standard (IS) strain was evaluated in a collaborative study involving 23 laboratories from 10 countries. The IS, code number 6329/10, was formulated by using a genotype 3a HEV strain from a blood donation, diluted in pooled human plasma and lyophilized. A Japanese national standard, representing a genotype 3b HEV strain, was prepared and evaluated in parallel. The potencies of the standards were determined by qualitative and quantitative assays. Assay variability was substantially reduced when HEV RNA concentrations were expressed relative to the IS. Thus, WHO has established 6329/10 as the IS for HEV RNA, with a unitage of 250,000 International Units per milliliter.

Published
2013
Full Text
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