1. Virus strain from a mild COVID-19 patient in Hangzhou represents a new trend in SARS-CoV-2 evolution potentially related to Furin cleavage site
- Author
-
Changzhong Jin, Jianhua Hu, Yue Ren, Guodong Yu, Hong-Yu Jia, Xiaoyan Wang, Yimin Zhang, Jifang Sheng, Dong Chen, Jian Shen, Dairong Xiang, Penglei Jiang, Yida Yang, Hangping Yao, Liang Wen, Xiangyun Lu, Jiangshan Lian, Nanping Wu, Shaorui Hao, Fumin Liu, Linfang Cheng, Yingfeng Lu, Jinmei Yao, Lanjuan Li, Liang Yu, Lin Zheng, Xiaopeng Yu, Kangli Xu, Haibo Wu, Xiaofeng Yang, Jianguo Gao, Xi Jin, Tingbo Liang, Yunqing Qiu, Pengxu Qian, and Nuoheng zheng
- Subjects
0301 basic medicine ,Adult ,Male ,China ,Epidemiology ,viruses ,030106 microbiology ,Pneumonia, Viral ,Immunology ,ACE2 ,Sequence alignment ,Gene mutation ,medicine.disease_cause ,Genome ,Microbiology ,Virus ,Evolution, Molecular ,03 medical and health sciences ,Betacoronavirus ,Phylogenetics ,Virology ,Drug Discovery ,medicine ,Humans ,Codon ,Furin ,Pandemics ,Phylogeny ,Coronavirus ,Retrospective Studies ,Genetics ,biology ,SARS-CoV-2 ,Sequence Analysis, RNA ,COVID-19 ,Articles ,General Medicine ,Middle Aged ,030104 developmental biology ,Infectious Diseases ,Codon usage bias ,Mutation ,biology.protein ,Disease Progression ,Female ,Parasitology ,Coronavirus Infections ,Research Article - Abstract
The mutations in the SARS-CoV-2 virus genome during COVID-19 dissemination are unclear. In 788 COVID-19 patients from Zhejiang province, we observed decreased rate of severe/critical cases compared with patients in Wuhan. For mechanisms exploration, we isolated one strain of SARS-CoV-2 (ZJ01) from a mild COVID-19 patient. Thirty-five specific gene mutations were identified. Phylogenetic and relative synonymous codon usage analysis suggested that ZJ01 may be a potential evolutionary branch of SARS-CoV-2. We classified 54 global virus strains based on the base (C or T) at positions 8824 and 28247 while ZJ01 has T at both sites. The prediction of the Furin cleavage site (FCS) and sequence alignment indicated that the FCS may be an important site of coronavirus evolution. ZJ01 mutations identified near the FCS (F1-2) caused changes in the structure and electrostatic distribution of the S surface protein, further affecting the binding capacity of Furin. Single-cell sequencing and ACE2-Furin co-expression results confirmed that the Furin expression was especially higher in glands, liver, kidneys, and colon. The evolutionary pattern of SARS-CoV-2 towards FCS formation may result in its clinical symptom becoming closer to HKU-1 and OC43 caused mild flu-like symptoms, further showing its potential in differentiating into mild COVID-19 subtypes.
- Published
- 2020
- Full Text
- View/download PDF