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Purposing to the thermal profile management of typical format 21700 lithium-ion battery cells, this study develops a cellular liquid cooling jacket to meet their requirements. To this end, the effects of fluid flowing, channel dimension, and cooling medium on the cell thermal profile were investigated numerically, and then the simulation effectiveness was authenticated experimentally as per a constructed test bench. Meanwhile, a non-dimensional analysis for the optimum design of cellular cooling jacket was implemented. The results show that the interlaced flow directions can capture a lower temperature standard deviation and a more even thermal distribution. The non-dimensional analysis reveals that the increasing fluid flow rate and channel size can effectively promote the performance of high-efficiency and low-impedance in the designed cellular cooling plate. The glycol aqueous solution to minimize cell’s temperature difference and temperature standard deviation is under a stronger capability than that of the liquid water. Experimental verification indicates that the developed cellular cooling jacket is of great control effectiveness to the thermal profile of the cylindrical 21700 cells. A benefit inside this study for the thermal management system designing for the power battery pack of composing with the cylindrical 21700 cells.