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Using energy conservation measures (ECMs) for building retrofit can significantly reduce building operational carbon emissions (BOCEs) and contribute to Chinese carbon neutrality by 2060. This paper involves 11 feasible ECMs for commercial buildings in hot-summer and cold-winter zone, China, and proposes a quick assessment approach to BOCEs reduction. Prototypical models are simulated for ECMs using EnergyPlus and the emission intensity is corrected by the building shape factor and window-to-wall ratio. Based on pre-simulated results, effects on reducing emissions of individual measures and packages of them are studied to develop regression models in SPSS. The results show that decreasing the U-factor of external walls, lighting power density (LPD), or shading coefficient can reduce BOCEs linearly. BOCEs are most sensitive to LPD and using daylighting control can reduce emissions by 5–15%. In addition, increasing the boilers' efficiency reduces emissions linearly, while performance improvement of chillers or heat pumps reduces emissions logarithmically. Furthermore, utilizing variable speed pumps, heat recovery ventilation, and air-side economizers can reduce emissions by 3.8%, 6.6%, and 15.2%, respectively. Finally, significant interactions exist among measures of enhancing glazing U-factor, adding external shading, using energy-saving lamps, and improving cooling/heating system efficiencies. The developed regression models are validated in a retrofit case in Shanghai. Through assessment, the case can reduce BOCEs by 15.9 kgCO 2 /m2, with a reduction rate of 20.8%. Compared with recorded data, the relative error of assessing emission reduction is −13.0% and that of assessing reduction rate is 0.5%, which are acceptable in the decision-making stage of retrofit strategies. [ABSTRACT FROM AUTHOR]