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Highlights • Influence of temperature fluctuations was examined in a human subjective experiment. • Generated airflows had distinct cooling effect on subjects. • Study indicates that sensation of draught was influenced by airflow temperature fluctuations. Abstract Besides air velocity, turbulence intensity and air temperature there are also other factors, which influence human thermal response to air movement. In this study, the impact of temperature fluctuations in airflow on human thermal sensation and perception of draught was examined. For the purposes of the study an air supply unit was designed for generating airflows with temperature fluctuations and used in a subjective experiment. The experimental study indicates that temperature fluctuations possibly influence the human perception of air movement with a distinct cooling effect. At high values of frequency and amplitude fluctuations a large discrepancy was found between predicted and measured values of draught discomfort. [ABSTRACT FROM AUTHOR]