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The EU Green Deal envisages a significant reduction in the use of industrial mineral fertilizers in agrotechnologies. The use of ash from another fuel made from plant biomass could help not only to utilize combustion residues, but also to optimize the nutrition of cultivated agricultural plants without harming the environment. With this aim, a pot experiment of the effects of multi-crops biomass ash on spring barley sprouts was carried out in January-February 2024 in the greenhouse of the Academy of Agriculture of the Vytautas Magnus University (VMU). Four ash fertilization rates were tested: 1. Unfertilized (N0, comparative-control treatment); 2. Fertilized at a low rate (N1); 3. Fertilized at an average rate (N2); 4. Fertilized at a high rate (N3). Or 200, 1000 and 2000 kg·ha-2, respectively. According to the results of investigations, the average rate of ash fertilization (N2) was the most effective for spring barley sprout growth. The shoot length was about 6 cm higher than in control. Ash fertilization rates had no significant effect on the root development. Fertilization with ash initiated the rise of the concentration of chlorophyll in spring barley leaves significantly by up to 46%. Fertilization with ash usually reduced the amount of dry matter in barley shoots but increased it in their roots. [ABSTRACT FROM AUTHOR]