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The Gutenberg-Richter Law describes the frequency-magnitude distribution of earthquakes. A number of studies have shown that the slope (b value) of the relationship between frequency and magnitude decreased before large earthquakes. In this paper, we investigate the temporal variation of the b value off the Pacific coast of Tokachi, Hokkaido, Japan, during 1990–2014. The magnitude of completeness (Mc) in the catalog is evaluated by combining the maximum curvature (MAXC) technique and the bootstrap approach. Then, the b value, and its uncertainty, is computed by using the maximum likelihood estimation. The Akaike Information Criterion (AIC) with the bootstrap approach is introduced to statistically assess the temporal variation of b values and quantify the significance level. The results show a decrease in trends of the b value prior to two large earthquakes (26 September 2003 (M8.0) and 11 September 2008 (M7.1)) in the analyzed area. In addition, the decrease of b values shows certain statistical significance three months before the 2003 Earthquake off the Pacific coast of Tokachi (M8.0). It is concluded that the b value with statistical assessment may contain potential information for future large earthquake preparation off the Pacific coast of Tokachi, Hokkaido, Japan. [ABSTRACT FROM AUTHOR]