1. Synthetic multispecies microbial communities reveals shifts in secondary metabolism and facilitates cryptic natural product discovery.
- Author
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Shi, Yutong, Pan, Chengqian, Wang, Kuiwu, Chen, Xuegang, Wu, Xiaodan, Chen, Chen ‐ Tung Arthur, and Wu, Bin
- Subjects
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MICROBIAL communities , *SECONDARY metabolism , *METABOLITES , *KLEBSIELLA pneumoniae , *BIOLOGICAL crosstalk - Abstract
Chemically mediated interactions have been hypothesized to be essential for ecosystem functioning as co-occurring organisms can influence the performance of each other by metabolic means. Here, we present a co-culture device that allows co-culturing of microorganisms that are physically separated but can exchange chemical signals and metabolites. This setup was adopted to perform investigations on the secondary metabolisms of both a fungal-bacterial community and an actinomycetic-actinomycetic community. This study employed a metabolomics approach integrating LC-MS profiling, multivariate data analysis and molecular networking techniques. LC-MS measurements revealed a pronounced influence of such chemical communication on the metabolic profiles of synthetic co-culture communities with a group of molecules being induced or upregulated in co-cultures. A novel antibiotic exhibiting antibiotic properties against Klebsiella pneumoniae was unveiled in the fungal-bacterial community. Besides, a further survey of the fungal-bacterial cross-talk indicated that the production of co-culture-induced diphenyl ethers by fungi might result from the fungal response against the secretion of surfactins by bacteria in the cross-talk. This study demonstrated that the presented co-culture device and the metabolomic routine would facilitate the investigation on chemically mediated interactions in nature as well as cryptic natural products discovery. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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