Uganda in East Africa is experiencing highly variable rainfall which is exacerbated by temperatures warming at faster rates. This study analyzed rainfall and temperature patterns in comparison with the potential evaporation transpiration rates (PETs) for parts of Central, Western, Southern, and Southwestern Uganda for varying periods from 1981 to 2022. For rainfall onset date (OD), threshold of 0.85 mm for a rainy day, rainfall of 20 mm accumulated over 5 days with at least 3 rain days, and dry spell not exceeding 9 days in the next 30 days were used. The rainfall cessation dates (RCDs) are determined when water balance (WB) falls below 5 mm in 7 days in the last month of the expected season (May and December) for the first and second season, respectively. Standardized rainfall anomaly was utilized to show seasonal and annual rainfall variability. Pearson's correlation (r) coefficient was used to show the relationship between weather variables (rainfall, temperature) and PET at five rainfall stations. Results showed highly varied onset and cessation dates for March-May (MAM) seasonal rainfall compared to those of September-December (SOND). Results showed highly variable onset and cessation of rainfall over the region and statistically significantly increasing trends in both maximum and minimum temperatures across the region, with the highest rate of increase of maximum and minimum temperature of 0.70 and 0.65 °C per decade respectively. Moreover, the maximum temperature and PET showed strong positive correlation coefficient (r) that ranged from 0.76 to 0.90 across the regions, which likely contribute to excess evaporation from the surfaces, soil moisture deficits that negatively affect plant biomass production, low crop yields and food insecurity. PET and rainfall revealed insignificant statistical negative correlation as indicated by the correlation coefficient ranging from - 0.04 to - 0.22. We recommend water management and conservation practices such as mulching, zero tillage, agroforestry, planting drought-resistant crops, and using affordable irrigation systems during period of water deficit., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)