Search

The presence of both chlorine-resistant bacteria (CRB) and microplastics (MPs) in drinking water distribution systems (DWDS) poses a threat to water quality and human health. However, the risk of CRB bio evolution under the stress of MPs remains unclear. In this study, polypropylene (PP) and polyethylene (PE) were selected to study the adsorption and desorption behavior of sulfamethoxazole (SMX), and it was clear that MPs had the risk of carrying pollutants into DWDS and releasing them. The results of the antibiotic susceptibility test and disinfection experiment confirmed that MPs could enhance the resistance of CRB to antibiotics and disinfectants. Bacteria epigenetic resistance mechanisms were approached from multiple perspectives, including physiological and biochemical characteristics, as well as molecular regulatory networks. When MPs enter DWDS, CRB could attach to the surface of MPs and directly interact with both MPs and the antibiotics they release. This attachment process promoted changes in the composition and content of extracellular polymers (EPS) within cells, enhanced surface hydrophobicity, stimulated oxidative stress function, and notably elevated the relative abundance of certain antibiotic resistance genes (ARGs). This study elucidates the mechanism by which MPs alter the intrinsic properties of CRB, providing valuable insights into the effective avoidance of biological risks to water quality during CRB evolution. [Display omitted] • Chlorine disinfection was deteriorated by the presence of MPs. • MPs contributed to the upregulation of multi-antibiotic resistance genes in CRB. • Oxidative stress response was triggered by MPs. [ABSTRACT FROM AUTHOR]

The antibiotic-resistant pollution in size-segregated bioaerosols from wastewater treatment plants (WWTPs) is of increasing concern due to its public health risks, but an elaborate review is still lacking. This work overviewed the profile, mobility, pathogenic hosts, source, and risks of antibiotic resistance genes (ARGs) in size-segregated bioaerosols from WWTPs. The dominant ARG type in size-segregated bioaerosols from WWTPs was multidrug resistance genes. Treatment units that equipped with mechanical facilities and aeration devices, such as grilles, grit chambers, biochemical reaction tanks, and sludge treatment units, were the primary sources of bioaerosol antibiotic resistome in WWTPs. Higher enrichment of antibiotic resistome in particulate matter with an aerodynamic diameter of <2.5 μm, was found along the upwind-downwind-WWTPs gradient. Only a small portion of ARGs in inhalable bioaerosols from WWTPs were flanked by mobile genetic elements. The pathogens with multiple drug resistance had been found in size-segregated bioaerosols from WWTPs. Different ARGs or antibiotic resistant bacteria have different aerosolization potential associated with bioaerosols from various treatment processes. The validation of pathogenic antibiotic resistance bacteria, deeper investigation of ARG mobility, emission mechanism of antibiotic resistome, and development of treatment technologies, should be systematically considered in future. [Display omitted] • Antibiotic resistome in size-segregated bioaerosols from WWTPs was overviewed. • Mobility and pathogenic hosts in WWTP bioaerosols were discussed. • Wastewater or sludge was the major source for ARGs and ARB in WWTP bioaerosols. • Resistome risk of size-segregated bioaerosols from WWTPs was presented. • Expectations of antibiotic resistance in WWTP bioaerosols were proposed. [ABSTRACT FROM AUTHOR]

The application of compost in agriculture has led to the accumulation of antibiotic resistance genes (ARGs) and heavy metal resistance genes (MRGs) in the soil environment. In this study, the response of ARGs and MRGs to bamboo charcoal (BC) and bamboo vinegar (BV) during aerobic composting was investigated. Results showed that BC + BV treatment reduced the abundances of ARGs and mobile genetic elements (MGEs) during the thermophilic period, as well as achieved the lowest rebound during the cooling period. BC + BV promoted the growth of Firmicutes, thereby facilitating the thermophilic period of composting. The rebound of ARGs and MGEs can be explained by increasing the abundance of Actinobacteria and Proteobacteria at the end of composting. Composting reduced the abundances of MRGs comprising pcoA , tcrB , and cueO , whereas cusA and copA indicated the selective pressure imposed by heavy metals on bacteria. The fate of ARGs was mainly driven by MGEs, and heavy metals explained most of the variation in MRGs. Interestingly, nitrogen conversion also had an important effect on ARG and MRG profiles. Our current findings suggest that the addition of BC + BV during compost preparation is an effective method in controlling the mobility of ARGs and MRGs, thereby reducing the environmental problems. Image 1 • BC plus BV treatment showed the highest reduction of ARGs and MGEs. • Nitrogen conversion had an important effect on ARG and MRG profiles. • Fate of ARGs was mainly driven by MGEs. • cusA and copA confirmed the selection pressure of heavy metals. [ABSTRACT FROM AUTHOR]

Vermicomposting is a green technology used in the recycling of sewage sludge using the joint action of earthworms and microorganisms. Although tetracycline is present in abundance in sewage sludge, little attention has been given to its influence on vermicomposts. This study investigated the effects of different tetracycline concentrations (0, 100, 500 and 1000 mg/kg) on the decomposition of organic matter, microbial community and antibiotic resistance genes (ARGs) during vermicomposting of spiked sludge. The results showed that 100 mg/kg tetracycline could stimulate earthworms' growth, accompanied by the highest humification and decomposition rates of organic matter in the sludge. The abundance of active microbial cells and diversity decreased with the increase in tetracycline concentrations. The member of Bacteroidetes dominated in the tetracycline spiked treatments, especially in the higher concentration treatments. Compared to its counterparts, the addition of tetracycline significantly increased the abundances of ARGs (tet C, tet M, tet X, tet G and tet W) and Class 1 integron (int -1) by 4.7–186.9 folds and 4.25 folds, respectively. The genera of Bacillus and Mycobacterium were the possible bacterial pathogen hosts of ARGs enriched in tetracycline added group. This study suggests that higher concentration of tetracycline residual can modify microbial communities and increase the dissemination risk of ARGs for final sludge vermicompost. Image 1 • Effects of tetracycline residuals on vermicomposting of sludge were studied. • 100 mg/kg tetracycline added in sludge showed the best humification level. • Microbial number and diversity were inhibited by higher content of tetracycline. • Tetracycline residuals enriched the abundances of ARGs and Int 1 in vermicompost. • Tetracycline should be controlled within 100 mg/kg before vermicomposting. Low concentration of tetracycline could significantly stimulate humification of sludge but enhance the abundances of ARGs and their dissemination risk during vermicomposting. [ABSTRACT FROM AUTHOR]

Composting is usually employed to treat livestock manure, and inoculation with lignocellulose-degrading microorganisms can enhance the quality of compost. In this study, lignocellulose-degrading microorganisms were inoculated at two levels (uninoculated control = 0%, and T treatment = 10%) during co-composting of swine manure with spent mushroom substrate, and their effects on antibiotic resistance genes (ARGs) and the bacterial community were investigated. Inoculation with lignocellulose-degrading microorganisms caused greater decreases in 6/11 ARGs and 3/4 mobile genetic elements than the control. The total relative abundances of ARGs increased by 0.23 logs in the control but decreased by 0.08 logs in the T treatment after co-composting. The bacterial community was clustered according to the composting time in the two treatments, where inoculation mainly affected the bacterial community during the mesophilic phase. Redundancy analysis and network analysis showed that the bacterial community succession had important effects on the variations in ARGs. Inoculation with lignocellulose-degrading microorganisms led to the reduction of ARGs, which was significantly correlated with the abundances of potential host bacteria for ARGs. Thus, inoculation with lignocellulose-degrading microorganisms could decrease the risk of ARGs spreading and make compost products more security. Image 1 • Inoculation with microorganisms led to greater decreases in 6/11 ARGs and 3/4 MGEs. • Inoculation with microorganisms affected bacterial community in mesophilic phase. • Bacterial community structure had important effects on the variations in ARGs. • Inoculation with microorganisms could decrease the risk of ARGs in compost product. Inoculation with lignocellulose-degrading microorganisms during co-composting could reduce ARGs, inhibit pathogens, and make compost products safer. [ABSTRACT FROM AUTHOR]

Land application of animal manure could change the profiles of antibiotic resistant bacteria (ARB), antibiotic resistance genes (ARGs) and bacterial communities in receiving soils. Using high-throughput real-time quantitative PCR and 16S rRNA amplicon sequencing techniques, this study investigated the ARGs and bacterial communities in field soils under various crop (corn and pasture) and manure (swine and dairy) managements, which were compared with those of two non-manured reference soils from adjacent golf course and grassland. In total 89 unique ARG subtypes were found in the soil samples and they conferred resistance via efflux pump, cellular protection and antibiotic deactivation. Compared to the ARGs in the golf course and grassland soils (28 and 34 subtypes respectively), manured soils generally had greater ARG diversity (36–55 subtypes). Cornfield soil frequently receiving raw swine manure had the greatest ARG abundance. The short-term (one week) application of composted and liquid swine manures increased the diversity and total abundance of ARGs in cornfield soils. Intriguingly the composted swine manure only marginally increased the total abundance of ARGs, but substantially increased the number of ARG subtypes in the cornfield soils. The network analysis revealed three major network modules in the co-occurrence patterns of ARG subtypes, and the hubs of these major modules (intl1-1 , vanC , and pncA) may be candidates for selecting indicator genes for surveillance of ARGs in manured soils. The network analyses between ARGs and bacteria taxa revealed the potential host bacteria for the detected ARGs (e.g., aminoglycoside resistance gene aacC4 may be mainly carried by Acidobacteriaceae). Overall, this study highlighted the potentially varying impact of various manure management on antibiotic resistome and microbiome in cornfield and pasture soils. Image 1 • Total 89 unique ARGs were detected in manured cornfield and pasture soils. • Manured soils generally had greater ARG diversity than non-manured soils. • Intl1-1 , vanC , and pncA may be candidates of indicator genes for monitoring ARGs. Manured soils generally had greater ARG diversity than non-manured soils. intl1-1 , vanC , and pncA may be candidates of indicator genes for monitoring ARGs in manured soils. [ABSTRACT FROM AUTHOR]

Abstract Coexistence of antibiotics/heavy metals and the overexpression of resistance genes in the vermicompost has become an emerging environmental issue. Little is known about the interaction and correlation between chemical pollutants and biological macromolecular compounds. In this study, three typical vermicompost samples were selected from the Yangtze River Delta region in China to investigate the antibiotic, heavy metal and corresponding antibiotic resistance genes (ARGs) and heavy metal resistance genes (HRGs). The results indicated the prevalence of tetracycline (TC), copper (Cu), zinc (Zn), cadmium (Cd), corresponding TC-resistance genes (tet A, tet C, tet W, tet M, tet O, and tet S) and HRGs (cop A, pco A, cus A, czc A, czc B, and czc R) in the three vermicompost samples. In addition, the ARG level was positively associated with the water-soluble TC fraction in the vermicompost, and it was same between the HRG abundance and exchangeable heavy metal content (p < 0.05). Moreover, a positive correlation was found between ARG and HRG abundance in the vermicompost samples, suggesting a close regulation mechanism involving the expression of both genes. The result obtained here could provide new insight into the controlling risk of heavy metals, TC, and relevant resistance genes mixed contamination in the vermicompost. Graphical abstract Image 1 Highlights • Tetracycline, heavy metals, and resistance genes co-existed in vermicomposts. • Antibiotic resistance gene level positively associated with the water-soluble TC fraction. • Heavy metal resistance gene level positively correlated with the exchangeable heavy metal species. • Significant positive correlation was observed between antibiotic- and heavy metal-resistance genes. Tetracycline, heavy metals, and associated resistance genes co-existed and positively correlated with each other in typical vermicomposts from Yangtze River Delta region in China. [ABSTRACT FROM AUTHOR]

Abstract Soil contamination with antibiotics and antibiotic resistant bacteria/genes (ARB/ARGs) has becoming an emerging environmental problem. Moreover, the mixed pollutants' transfer and accumulation from soil to tuberous vegetables has posed a great threat against food security and human health. In this work, the application of two absorbing materials (maize biochar and sulfate modified eggshell) was able to reduce the poisonous effect of soil antibiotics on potato root system by stimulate the dissipation of water-soluble antibiotics in soil; and also improve food quality by increasing potato starch, protein, fat, and vitamins. Meanwhile, both amendments could effectively decrease the classes and the accumulative abundance of ARB and ARGs (sul I, sul II, cat I, cat II, erm A, erm B) in the edible parts of potato. The lowest abundance of ARGs was detected in the biochar application treatment, with the accumulative ARG level of 8.9 × 102 and 7.2 × 102 copies mL−1 in potato peel (sul l + cat I + erm A) and tuberous root (sul I), respectively. It is the first study to demonstrate the feasibility of biochar and eggshell derived from agricultural wastes as green absorbing materials to reduce soil antibiotic, ARB, and ARGs accumulation risk in tuberous vegetable. Graphical abstract Image 1 Highlights • Biochar or eggshell impeded soil antibiotics accumulation in potato effectively. • ARB isolates in potato decreased significantly after biochar or eggshell addition. • ARG accumulation risk was limited in potato with biochar or eggshell amendment. • Impedance effect of biochar was more effective than that of eggshell. • Biochar or eggshell application provided new strategy to ensure vegetable security. ARB and ARG accumulation risk was limited in potato with biochar or eggshell amendment; Impedance effect of biochar was more effective than that of eggshell. [ABSTRACT FROM AUTHOR]

Wastewater Treatment Plant (WWTP) effluents are important sources of antibiotics, antibiotic resistance genes (ARGs) and resistant bacteria that threaten aquatic biota and human heath. Antibiotic effects on host-associated microbiomes, spread of ARGs and the consequences for host health are still poorly described. This study investigated changes of the Daphnia magna associated microbiome exposed to the recalcitrant antibiotic doxycycline under artificial reconstituted lab water media (lab water) and treated wastewater media. D. magna individual juveniles were exposed for 10 days to treated wastewater with and without doxycycline, and similarly in lab water. We analysed 16 S rRNA gene sequences to assess changes in community structure, monitored Daphnia offspring production and quantified ARGs abundances by qPCR from both Daphnia and water (before and after the exposure). Results showed that doxycycline and media (lab water or wastewater) had a significant effect modulating Daphnia -associated microbiome composition and one of the most discriminant taxa was Enterococcus spp. Moreover, in lab water, doxycycline reduced the presence of Limnohabitans sp., which are dominant bacteria of the D. magna- associated microbiome and impaired Daphnia reproduction. Contrarily, treated wastewater increased diversity and richness of Daphnia -associated microbiome and promoted fecundity. In addition, the detected ARG genes in both lab water and treated wastewater medium included the qnrS1 , sul 1, and bla TEM, and the integron-related int I1 gene. The treated wastewater contained about 10 times more ARGs than lab water alone. Furthermore, there was an increase of sul 1 in Daphnia cultured in treated wastewater compared to lab water. In addition, there were signs of a higher biodegradation of doxycycline by microbiomes of treated wastewater in comparison to lab water. Thus, results suggest that Daphnia -associated microbiomes are influenced by their environment, and that bacterial communities present in treated wastewater are better suited to cope with the effects of antibiotics. [Display omitted] • Wastewater and antibiotic exposures changed the Daphnia magna microbiome. • Antibiotics decreased Daphnia fecundity and changed microbiome composition. • Daphnia individuals were able to differentially accumulate sul 1 from waste water. • Specific bacteria genera from the Daphnia microbiome could carry sul 1. [ABSTRACT FROM AUTHOR]

Soil solarization (SS) is a technique for managing pathogens and weeds, which involves covering with transparent plastic to increase soil temperature during summer fallow (SF). However, SS also alters the diversity of bacterial communities. Therefore, during SF, various organic modifiers are used in combination with SS to improve its efficacy. Organic amendments may contain antibiotic resistance genes (ARGs). Greenhouse vegetable production (GVP) soils are vital to ensure food security and ecological balance. However, comprehensive study on the effects of SS combined with different types of manure on ARGs in GVP soils during SF remains unclear. Therefore, this study employed high-throughput qPCR to explore the effects of different organic amendments combined with SS on the abundance changes of ARGs and mobile genetic elements (MGEs) in GVP soils during SF. The abundance and diversity of ARGs and MGEs in GVP soils with different manure fertilization and SS decreased during SF. Horizontal gene transfer via MGEs (especially integrases 45.80%) induced by changes in environmental factors (NO 3 −-N 14.7% and NH 4 +-N) was the main factor responsible for the changes in ARGs. Proteobacteria (14.3%) and Firmicutes were the main potential hosts of ARGs. Network analysis suggested that Ornithinimicrobium , Idiomarina and Corynebacterium had positive correlations with aminoglycosides, MLSB, and tetracycline resistance genes. These results provide new insights to understand the fate of ARGs in the GVP soils by manure-amended combined with SS during SF, which may help to reduce the spread of ARGs. [Display omitted] • SS combined with different manures application alleviated the ARGs transmission during SF. • During SF, BM mainly reduced Multidrugs, while CM reduced Beta_ Lactamase (21). • HGT via MGEs contributed more to ARGs abundance than physicochemical properties and microbial. • Potential hosts of ARGs mainly belonged to Proteobacteria and Firmicutes. [ABSTRACT FROM AUTHOR]

The transfer of antibiotic resistance genes (ARGs) in the environment is a threat to both human and animal health. However, the contribution of bacteriophages to the dissemination of resistance genes via transduction is rarely explored. In this study, we screened pig feces from three commercial farms in China for 32 clinically relevant ARG types to assess the presence of the ARG population in bacteria and bacteriophage and further to estimate the contribution of bacteriophages to the dissemination of antibiotic resistance. We found that bacteriophage DNA contained 35.5% of the target ARG types and sul1 , bla TEM and ermB were found in 100% of the phage DNA samples. The most abundant genes in the bacterial population were ermB and fexA whereas ermB was the most abundant in bacteriophage. In contrast, floR was the least abundant ARG in both populations. Also, the ratio index of the abundance of ARGs in bacteriophage and bacteria was firstly used in this study as an estimator of bacteriophage ability to transmit ARGs. The ratio for qnrA was the greatest (about 10 −1 ) and differed from the most abundant bacteriophage ARG ermB. In addition, fexA had the lowest ratio value (about 10 −6 ) and not floR . These results illustrate that ARGs abundance and detection rates used alone probably be not suitable for comprehensively judging the contribution of bacteriophage to the dissemination of antibiotic resistance. A more suitable model is the application of three indices; occurrence rate, absolute abundance in bacteriophage and the ratio value as warning and monitoring tools for environmental ARG assessments in bacteriophages. [ABSTRACT FROM AUTHOR]

Antibiotic resistance is an increasingly important environmental pollutant with direct consequences for human health. Identification of environmental sources of antibiotic resistance genes (ARGs) makes it possible to follow their evolution and prevent their entry into the clinical setting. ARGs have been found in environmental sources exogenous to the original source and previous studies have shown that these genes are capable of being transferred from livestock to humans. Due to the nature of farming and the slaughter of ruminants for food, humans interact with these animals in close proximity, and for this reason it is important to consider the risks to human health. In this study, we characterised the ARG populations in the ovine rumen, termed the resistome. This was done using the Comprehensive Antibiotic Resistance Database (CARD) to identify the presence of genes conferring resistance to antibiotics within the rumen. Genes were successfully mapped to those that confer resistance to a total of 30 different antibiotics. Daptomycin was identified as the most common antibiotic for which resistance is present, suggesting that ruminants may be a source of daptomycin ARGs. Colistin resistance, conferred by the gene pmrE , was also found to be present within all samples, with an average abundance of 800 counts. Due to the high abundance of some ARGs (against daptomycin) and the presence of rare ARGs (against colistin), we suggest further study and monitoring of the rumen resistome as a possible source of clinically relevant ARGs. [ABSTRACT FROM AUTHOR]

Antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) can be identified with metagenomic analyses comparing relatively pristine and human-impacted environments. We collected samples from 3 different environments: glacial soil little affected by anthropogenic activity, deep permafrost dated to 5821 BP (before human antibiotics), and sediment from the Pearl River. Sulfonamides, tetracyclines, and fluoroquinolones were common in the sediment samples. Sulfonamides and tetracycline were not found in permafrost; tetracycline was also not found in glacial soil. ARGs from the sediment were more abundant and diverse than those from glacial soil and permafrost. More types of resistance mechanisms were also present in the sediment. The diversity of MGEs was significantly correlated with the abundance and diversity of ARGs. The result will help future workers to better understand the distribution of ARGs among environments more or less impacted by anthropogenic activities. [ABSTRACT FROM AUTHOR]

Poultry farming is a major livelihood in South and Southeast Asian economies where it is undergoing rapid intensification to meet the growing human demand for dietary protein. Intensification of poultry production systems is commonly supported by increased antimicrobial drug use, risking greater selection and dissemination of antimicrobial resistance genes (ARGs). Transmission of ARGs through food chains is an emerging threat. Here, we investigated transmission of ARGs from chicken (broiler and layer) litter to soil and Sorghum bicolor (L.) Moench plants based on field and pot experiments. The results demonstrate ARGs transmission from poultry litter to plant systems under field as well as experimental pot conditions. The most common ARGs could be tracked for transmission from litter to soil to plants were identified as detected were cmx , ErmX , ErmF , lnuB , TEM-98 and TEM-99 , while common microorganisms included Escherichia coli , Staphylococcus aureus , Enterococcus faecium , Pseudomonas aeruginosa , and Vibrio cholerae. Using next generation sequencing and digital PCR assays we detected ARGs transmitted from poultry litter in both the roots and stems of S. bicolor (L.) Moench plants. Poultry litter is frequently used as a fertiliser because of its high nitrogen content; our studies show that ARGs can transmit from litter to plants and illustrates the risks posed to the environment by antimicrobial treatment of poultry. This knowledge is useful for formulating intervention strategies that can reduce or prevent ARGs transmission from one value chain to another, improving understanding of impacts on human and environmental health. The research outcome will help in further understanding the transmission and risks posed by ARGs from poultry to environmental and human/animal health. [Display omitted] • Prevalence of AMR genes in poultry litter, soil and plant were reported. • Copy number of relevant AMR genes were validated using droplet digital PCR. • Campylobacter jejuni was found in samples sequenced from broiler and layer litters. • Dominant pathogens of origin were represented by Staphylococcus, Enterococcus etc. • Transmission of AMR genes to plants via soil have been demonstrated. [ABSTRACT FROM AUTHOR]

Antibiotics and antibiotic resistance genes (ARGs) in soil can affect human health via the food chain. Biochar is a soil amendment but its impacts on ARGs and the microbial communities associated with soil and vegetables are unclear. Therefore, we established three lettuce pot culture experiments, i.e., O300: 300 mg/kg oxytetracycline (OTC), BO300: 300 mg/kg OTC + 2% biochar, and a control without OTC or biochar. We found that under BO300, the relative abundances of ARGs were reduced by 51.8%, 43.4%, and 44.1% in lettuce leaves, roots, and soil, respectively, compared with O300. intI1 was highly abundant in soil and lettuce, and it co-occurred with some ARGs (tetW, ermF, and sul1). Redundancy analysis and network analysis indicated that the bacterial community succession was the main mechanism that affected the variations in ARGs and intI1. The reduction of Firmicutes due to the biochar treatment of soil and lettuce was the main factor responsible for the removal of tetracycline resistance genes in leaves. Biochar application led to the disappearance of human pathogenic bacteria (HPB), which was significantly correlated with the abundances of ermF and ermX. In summary, biochar is an effective farmland amendment for reducing the abundances of antibiotics, ARGs, and HPB in order to ensure the safety of vegetables and protect human health. [ABSTRACT FROM AUTHOR]

The prevalence of antibiotic resistance genes (ARGs), owing to irrigation using untreated swine wastewater, in vegetable-cultivated soils around swine farms poses severe threats to human health. Furthermore, at the field scale, the remediation of such soils is still challenging. Therefore, here, we performed field-scale experiments involving the cultivation of Brassica pekinensis in a swine wastewater-treated soil amended with composted pig manure, biochar, or their combination. Specifically, the ARG and mobile genetic element (MGE) profiles of bulk soil (BS), rhizosphere soil (RS), and root endophyte (RE) samples were examined using high-throughput quantitative polymerase chain reaction. In total, 117 ARGs and 22 MGEs were detected. Moreover, we observed that soil amendment using composted pig manure, biochar, or their combination decreased the absolute abundance of ARGs in BS and RE after 90 days of treatment. However, the decrease in the abundance of ARGs in RS was not significant. We also observed that the manure and biochar co-application showed a minimal synergistic effect. To clarify this observation, we performed network and Spearman correlation analyses and used structure equation models to explore the correlations among ARGs, MGEs, bacterial composition, and soil properties. The results revealed that the soil amendments reduced the abundances of MGEs and potential ARG-carrying bacteria. Additionally, weakened horizontal gene transfer was responsible for the dissipation of ARGs. Thus, our results indicate that composted manure application, with or without biochar, is a useful strategy for soil nutrient supplementation and alleviating farmland ARG pollution, providing a justification for using an alternative to the common agricultural practice of treating the soil using only untreated swine wastewater. Additionally, our results are important in the context of soil health for sustainable agriculture. [Display omitted] • Soil amendment by manure/biochar reduced antibiotic resistance gene (ARG) abundance. • Weakened horizontal gene transfer was responsible for ARG dissipation. • Co-application of composted manure and biochar had a minimal synergistic effect. [ABSTRACT FROM AUTHOR]

The amendment of organic fertilizer derived from livestock manure or biosolids is a significant driver of increasing antibiotic resistance in agricultural soils; however, it remains unclear whether increasing organic fertilizer application rates consistently enhances soil antibiotic resistance levels. Herein, we collected soils with long-term amendment with three types of organic fertilizers at four application rates (15, 30, 45, and 60 t/ha/y) and found that the higher the fertilization rate, the higher the antibiotic resistance gene (ARG) abundance. However, when the fertilization rate exceeded 45 t/ha/y, the ARG abundance ceased to significantly increase. Moreover, the soil ARG abundance was positively correlated with total nitrogen (TN) content and bacterial abundance, especially Firmicutes, and negatively affected by pH and bacterial diversity. Soil TN/bacterial abundance and pH/bacterial diversity reached maximum and minimum values at the 45 t/ha/y fertilization rate, respectively. Meanwhile, at this fertilization rate, Firmicutes enrichment peaked. Therefore, an organic fertilization rate of 45 t/ha/y appeared to represent the threshold for soil antibiotic resistance in this study. The underlying mechanism for this threshold was closely related to soil TN, pH, bacterial abundance, and diversity. Taken together, the findings of this study advance the current understanding regarding the soil resistome under different fertilization rates, while also providing novel insights into organic fertilizer management in agricultural practices. [Display omitted] • Organic fertilization rate of 45 t/ha/y is soil antibiotic resistance threshold. • Resistance threshold is related to soil TN, pH, bacterial abundance/diversity. • Soil ARG abundance positively correlates with TN and bacterial abundance. • Soil ARG abundance negatively correlates with pH and bacterial diversity. • Firmicutes abundance peaks at a 45 t/ha/y fertilization rate. [ABSTRACT FROM AUTHOR]

Antibiotic resistance genes (ARGs) and nanoplastics (NPs) have been identified as emerging pollutants in water environment; the interactions between antibiotic resistance plasmids (ARPs) and NPs will influence ARG transport in sediments. Herein, the adsorption experiments of a typical ARP onto polystyrene nanoplastics (PS-NPs) in river and lake sediments were conducted to elucidate the adsorption mechanisms and the effects of environmental factors. Results indicated that the adsorption amounts of PS-NPs increased with the dosages while decreased with the particle size of sediments. Multi-layer adsorption of PS-NPs was found to exist mainly in sand and silt sediments, whereas the filling adsorption dominated in the clay. Moreover, the adsorbed PS-NPs enhanced the physisorption of ARPs in sediments through stimulating the intraparticle diffusion of ARPs induced by electrostatic force. Besides, the adsorption amounts of ARPs onto the PS-NPs decreased with the increasing pH and dissolve organic matter due to the enhanced electrostatic repulsion and competitive adsorption. The ion strength played catalytic roles by increasing the electrostatic attraction and adsorption sites of ARPs on PS-NPs. The adsorbed ARPs in sediments were closely related with the ARGs in extra/intracellular DNA of biofilms, influencing the distribution and proliferation of ARGs largely. The findings indicate that ARG-associated pollution might be enhanced by the solid-liquid interface adsorption induced by NPs, which was controlled by pH, ion strength and dissolve organic matter. This study provides supplementary insights into the roles of NPs as carriers of ARP in sediments, and advances our understanding on the risks of NP-ARG co-occurring contamination in aquatic ecosystems. [Display omitted] • Nanoparticle adsorption was affected by sediment composition and particle size. • Nanoparticles enhanced ARP diffusion and affinity by electrostatic force. • PH, ion strength and the dissolved organic matter affected ARP adsorption. • Relative abundance of ARGs in biofilms was positively related to absorbed ARPs. [ABSTRACT FROM AUTHOR]

Contamination of antibiotic resistance genes (ARGs) from animals is a serious issue as they may spread to human pathogenic bacteria. The reduction of ARG contamination from livestock waste is thus essential. High solid anaerobic digestion (HSAD) is a new and effective technology although some aspects, such as change characteristics of ARGs at different reaction stages, have not been fully investigated. This study focused firstly on the variations in ARGs at different reaction stages in HSAD systems with five different starting methods: 1 natural start (the control) and 4 rapid starts by changing leachate reflux forms. The results showed that the rapid starting methods could accelerate start-up and increase biogas production by 312.5%. The starting and acidification stages showed higher microbial richness and diversity compared with the other stages. ARGs found to be reduced at acidification stage. Variation in ARGs at the starting and acidification stages was mainly driven by a combination of microbial community, mobile genetic elements (MGEs), and environmental factors; while the main contributory factors at the gas production stage were biomass and several unexplained factors. At the ending stage, the main driving factors were biomass and microbial communities. Most of the potential hosts (16/20) of the ARGs belonged to the Firmicutes phylum, which showed the lowest connections with the ARGs at the gas production stage. [Display omitted] • This study firstly focused on ARGs fate at different reaction stages. • ARGs and MGEs were mainly reduced at acidification stage. • The starting and acidification stage had higher microbial richness and diversity. • ARGs variation was driven by different factors at different stages. [ABSTRACT FROM AUTHOR]

Swine farm and the adjacent farmland are hot spots of ARGs. However, few studies have investigated the on-site occurrence of ARGs distributed in the process of anaerobic digestion (AD) followed by land application of swine wastewater. Two typical swine farms, in southern and northern China respectively, with AD along with land application were explored on ARG distributions. ARGs were highly abundant in raw swine wastewater, AD effectively reduced the copy number of all detected ARGs (0.21–1.34 logs removal), but the relative abundance with different resistance mechanisms showed distinctive variation trends. The reduction efficiency of ARGs was improved by stable operational temperature and longer solid retention time (SRT) of AD. ARGs in soil characterized the contamination from the irrigation of the digested liquor. The total ARGs quantity in soil fell down by 1.66 logs in idle period of winter compared to application period of summer in the northern region, whereas the total amount was steady with whole-year application in south. Some persistent ( sul 1 and sul 2) and elevated ARGs ( tet G and ere A) in AD and land application need more attention. [ABSTRACT FROM AUTHOR]

The effluents from wastewater treatment plants have been recognized as a significant environmental reservoir of antibiotics and antibiotic resistance genes (ARGs). Reclaimed water irrigation (RWI) is increasingly used as a practical solution for combating water scarcity in arid and semiarid regions, however, impacts of RWI on the patterns of ARGs and the soil bacterial community remain unclear. Here, we used high-throughput quantitative PCR and terminal restriction fragment length polymorphism techniques to compare the diversity, abundance and composition of a broad-spectrum of ARGs and total bacteria in 12 urban parks with and without RWI in Victoria, Australia. A total of 40 unique ARGs were detected across all park soils, with genes conferring resistance to β-lactam being the most prevalent ARG type. The total numbers and the fold changes of the detected ARGs were significantly increased by RWI, and marked shifts in ARG patterns were also observed in urban parks with RWI compared to those without RWI. The changes in ARG patterns were paralleled by a significant effect of RWI on the bacterial community structure and a co-occurrence pattern of the detected ARG types. There were significant and positive correlations between the fold changes of the integrase intI1 gene and two β-lactam resistance genes (KPC and IMP-2 groups), but no significant impacts of RWI on the abundances of intI1 and the transposase tnpA gene were found, indicating that RWI did not improve the potential for horizontal gene transfer of soil ARGs. Taken together, our findings suggested that irrigation of urban parks with reclaimed water could influence the abundance, diversity, and compositions of a wide variety of soil ARGs of clinical relevance. One-sentence summary Irrigation of urban parks with treated wastewater significantly increased the abundance and diversity of various antibiotic resistance genes, but did not significantly enhance their potential for horizontal gene transfer. [ABSTRACT FROM AUTHOR]

Antibiotic resistance genes (ARGs) and virulence factors (VFs) are critical threats to human health. Their abundance in aquatic ecosystems is maintained and enhanced via selection driven by environmental xenobiotics. However, their activity and expression in these environments under xenobiotic stress remains unknown. Here ARG and VF expression profiles were examined in aquatic microcosms under ciprofloxacin, glyphosate and sertraline hydrochloride treatment. Ciprofloxacin increased total expression of ARGs, particularly multidrug resistance genes. Total expression of ARGs and VFs decreased significantly under glyphosate and sertraline treatments. However, in opportunistic human pathogens, these agents increased expression of both ARGs and VFs. Xenobiotic pollutants, such as the compounds we tested here, have the potential to disrupt microbial ecology, promote resistance, and increase risk to human health. This study systematically evaluated the effects of environmental xenobiotics on transcription of ARGs and VFs, both of which have direct relevance to human health. Transcription of such genes has been overlooked in previous studies. [Display omitted] • ARGs and VFs were selectively expressed under different xenobiotic treatments. • Ciprofloxacin upregulated ARGs that confer resistance to wider range of antibiotics. • Glyphosate and sertraline hydrochloride induced ARGs and VFs expression in pathogens. • ARGs and VFs were co-expressed in the aquatic microbial community. • Changes of ARGs and VFs under xenobiotic treatments disrupted ecological functions. [ABSTRACT FROM AUTHOR]

Estuarine environments faced with contaminations from coastal zones and the inland are vital sinks of antibiotic resistance genes (ARGs). However, little is known about the temporal-spatial pattern of ARGs and its predominant constraints in estuarine environments. Here, we leveraged metagenomics to investigate ARG profiles from 16 China's estuaries across 6 climate zones in dry and wet seasons, and disentangled their relationships with environmental constraints. Our results revealed that ARG abundance, richness, and diversity in dry season were higher than those in wet season, and ARG abundance exhibited an increasing trend with latitude. The prevalence of ARGs was significantly driven by human activities, mobile gene elements, microbial communities, antibiotic residuals, physicochemical properties, and climatic variables. Among which, climatic variables and human activities ranked the most important factors, contributing 44% and 36% of the total variance of observed ARGs, respectively. The most important climatic variable shaping ARGs is temperature, where increasing temperature is associated with decreased ARGs. Our results highlight that the prevalence of ARGs in estuarine environments would be co-driven by anthropogenic activities and climate, and suggest the dynamics of ARGs under future changing climate and socioeconomic development. [Display omitted] • Metagenomics is used to explore ARG profiles in China's estuaries. • The abundance, richness, and diversity in dry season exceeds those in wet season. • The leading driver of ARGs on large scale is climate and human activities. • ARG abundance exhibits a significant upward with latitude. • Climate warming would result in decreased ARGs. [ABSTRACT FROM AUTHOR]

The spreading of extended-spectrum β-lactamases (ESBL)-producing thermotolerant coliforms (TC) in the water environment is a threat to human health but little is known about ESBL-producing TCs in the Yangtze River. We received 319 ESBL-producing stains obtained from the Chongqing basin and we investigated antibiotic susceptibility, bla gene types and the presence of integrons and gene cassettes. 16.8% of TC isolates were ESBL-producing bacteria and bla TEM+CTx-M was the predominant ESBL type. 65.2% of isolates contained class 1 integrons, but only 3 carried intI 2. Gene cassettes were amplified and sequenced. aadA, drfA, cmlA, sat1, aar3 and two ORF cassettes were found. In conclusion, Yangtze River is heavily polluted by ESBL-producing TC bacteria and the combined bla gene type could enhance antibiotic resistance. Class 1 integrons were widespread in ESBL-producing isolates and play an important role in multi-drug resistance. Characterization of gene cassettes could reveal the dissemination of antibiotic resistance genes. [Copyright &y& Elsevier]

Aquaculture ecosystem is a hot-spot for antibiotic resistance genes (ARGs). Rice-crayfish co-culture was considered an eco-friendly aquaculture model and has been widely adopted in China. However, it is unclear whether rice-crayfish co-culture is one of the most eco-friendly models from the perspective of antibiotic resistance profiles. In this study, we evaluated the eco-friendliness of rice-crayfish co-culture, and compared this model with other aquaculture models, from the perspectives of antibiotics and ARG patterns, based on multi-omics and antibiotic profiles. Results showed that the nutrient levels, antibiotic concentrations, dominant microbial genera and ARG patterns in the rice-crayfish co-culture model were profoundly different from the other three aquaculture models (crab only aquaculture model, crayfish only aquaculture model, and crab-crayfish co-culture models). Specifically, the rice-crayfish co-culture model has significantly lower diversity of ARGs and lower potential risks of ARGs when compared to the other aquaculture models. Nutrient and antibiotic concentrations were the important environmental factors for shaping ARG patterns, but compared with environmental factors, the effects of mobile genes and bacteria community on the proliferation and transmission of ARGs were stronger. This study has deepened our understanding of ARGs in freshwater aquaculture ecosystem, and suggested that rice-crayfish co-culture model is a relatively eco-friendly aquaculture model when compared with the other aquaculture models. [Display omitted] • Antibiotic resistome profiles in various aquaculture ecosystems were investigated. • Rice-crayfish co-culture model has lower ARG diversity and potential risks. • Main ARG-host bacteria varied in different sampling environments. • Nutrients and antibiotics were important driving factors for ARG profiles. • Rice-crayfish co-culture model is a more eco-friendly aquaculture model. [ABSTRACT FROM AUTHOR]

Coastal tourist and industrial cities are most likely to have differential effects on the distance dilution of antibiotic resistance genes (ARGs) in an estuary system. This study used high-throughput fluorescence quantitative polymerase chain reaction to identify sediment ARGs in two typical estuaries of coastal tourist and industrial cities (Xiamen and Taizhou) in China. The distance dilution of ARGs and its relationship with key environmental factors were analysed. The results indicated that along the river inlet towards the sea, the distance dilution effect on ARG abundance in estuary sediments of Taizhou was approximately double that in Xiamen, and the macrolide, lincosamide, and streptogramin B (MLSB) and vancomycin genes were replaced by the fluoroquinolone, quinolone, florfenicol, chloramphenicol, and amphenicol (FCA) and β-lactam genes in Taizhou, whereas β-lactam genes succeeded the MLSB and sul genes in Xiamen. The abundance and number of ARGs and mobile genetic elements (MGEs) were positively correlated with the particle size and total organic carbon (TOC) contents of sediments, whereas they were negatively associated with the oxidation and reduction potential (E h) and pH of sediments, as well as the seawater salinity. The sediment particle size (SPZ) was the dominant physicochemical factor affecting the abundance of ARGs (r = 0.826, p < 0.05) and MGEs (r = 0.850, p < 0.01). These findings suggest that although the distance dilution effect on the ARG abundance of estuary sediments of the industrial city is greater than that of the tourist city, the larger SPZ, higher TOC content, and lower salinity, pH, and E h in estuary regions adjacent to the industrial city can more significantly facilitate the proliferation and propagation of ARGs in the sediments. [Display omitted] • Distance dilution of estuarine ARGs in industrial city exceeded that of tourist city. • From river inlet to the sea, β-lactam ARGs replaced MLSB ARGs in estuary sediments. • Sediment particle size was the dominant physicochemical factor for the ARG abundance. • Salinity, oxidation and reduction potential, and pH were negatively related to ARGs. [ABSTRACT FROM AUTHOR]

The behaviors of typical veterinary antibiotics (oxytetracycline, ciprofloxacin and sulfamethazine) and 75 types of corresponding antibiotic resistant genes (ARGs) in four substrate systems (zeolite, gravel, red brick, and oyster shell) were investigated in this study. The results indicated that during treating synthetic livestock wastewater with individual antibiotic influent concentration of 100 μg/L, the effluent contained oxytetracycline and ciprofloxacin concentrations of 0.7–1.5 μg/L and 1.0–1.9 μg/L, respectively, in the zeolite and red brick systems, which were significantly lower than those of the other substrate systems (4.6–14.5 μg/L). Statistical correlation analyses indicated that the difference regarding oxytetracycline and ciprofloxacin removal among the four substrates was determined by their adsorption capacity which was controlled by the chemisorption mechanism. The average removal efficiency of sulfamethazine in the gravel system (48%) was higher than that of the other substrate systems (34–45%), and biodegradation may alter the sulfamethazine performance because of its co-metabolism process. Although tet G, flo R, sul 1, and qac EΔ1 were the dominant ARGs in all substrate systems (8.74 × 10−2-6.34 × 10−1), there was difference in the total ARG enrichment levels among the four substrates. Oyster shell exhibited the lowest total relative abundance (1.56 × 100) compared to that of the other substrates (1.82 × 100–2.27 × 100), and the ARG total relative abundance exhibited significant negative and positive correlations with the substrate pH and system bacterial diversity (P < 0.05), respectively. In summary, this study indicated that due to the difference of adsorption capacity and residual abundant nutrient in wastewater, the wetland substrate selection can affect the removal efficiency of veterinary antibiotics, and antibiotics may not be the determining factor of ARG enrichment in the substrate system. Image 1 • Wetland substrate selection can affect the veterinary antibiotic removal efficiency. • Adsorption capacity of substrate and co-metabolism determine antibiotic removal. • Antibiotic may not be the determining factor of ARG enrichment in substrate system. • ARG abundance is significantly related to substrate pH and bacterial diversity. [ABSTRACT FROM AUTHOR]

Mcr-1 and bla NDM-1 antibiotic resistance genes (ARGs) confer resistance to colistins and carbapenems, which are often antibiotics used as a last resort in tertiary care hospitals. Dissemination of these two ARGs in drinking water supply systems and their effect on healthy gut bacteria are poorly studied. In this study, the dissemination of mcr-1 and bla NDM-1 in a drinking water supply system, and their effect on the antibiotic resistance of mouse gut bacteria are explored. Metagenome analysis revealed that source water (Taipu river and Jinze reservoir) was polluted with ARGs. Mcr-1 and bla NDM-1 can be disseminated through the water distribution system. Even advanced water treatments (ozone and biological activated carbon (BAC)) could not effectively remove mcr-1 and bla NDM-1. Low concentrations of chloramine disinfectants in the water distribution system were not effective at limiting ARG abundance. Mobile genetic elements were also found to play a major role in the dissemination of ARGs via horizontal gene transfer (HGT) throughout the water supply system. Statistical analysis revealed that there was no effect of temperature on the abundance of mcr-1 and bla NDM-1 throughout the water supply system. A last resort ARG, mcr-1 can disseminate from drinking water to the healthy mouse gut. The presence of mcr-1 in a strain belonging to Enterococcus hirae , which is different from the strain belonging to the Bacillus cereus group isolated from drinking water, strongly supports the phenomena of HGT inside the gut. This research provides novel insights into the role of drinking water in disseminating ARGs to the gut and strongly suggests that drinking water may also play a major role apart from other factors known to be involved in the prevalence of last resort ARGs in the gut. Image 1 • mcr-1 and bla NDM-1 were found to disseminate from source to tap in water supply system. • Advanced water treatment process could not effectively remove mcr-1 and bla NDM-1. • Chloramine disinfection had no effect on elimination of mcr-1 and bla NDM-1. • Last resort ARG mcr-1 could disseminate from drinking water to the gut via HGT. • Drinking water is one of the reasons of last resort ARGs transfer in a healthy gut. mcr-1 and bla NDM-1 can disseminate through drinking water supply systems and mcr-1 was found to disseminate from drinking water to the mouse gut. [ABSTRACT FROM AUTHOR]

Long-term substantial application of fungicides in greenhouse cultivation led to residual pollution in soils and then altered soil microbial community. However, it is unclear whether residual fungicides could affect the diversity and abundance of antibiotic resistance genes (ARGs) in greenhouse soils. Here, the dissipation of fungicides and its impact on the abundance of ARGs were determined using shotgun metagenomic sequencing in the greenhouse and mountain soils under laboratory conditions. Our results showed the greenhouse soils harbored more diverse and abundant ARGs than the mountain soils. The application of carbendazim, azoxystrobin, and chlorothalonil could increase the abundance of total ARGs in the greenhouse soils, especially for those dominant ARG subtypes including sul 2, sul 1, aad A, tet (L), tet A(G), and tet X2. The abundant ARGs were significantly correlated with mobile genetic elements (MGEs, e.g. int I 1and R485) in the greenhouse soils but no significant relationship in the mountain soils. Meanwhile, the co-occurrence patterns of ARGs and MGEs, e.g., sul 2 and R485, sul 1 and transposase, were further verified via the genetic arrangement of genes on the metagenome-assembled contigs in the greenhouse soils. Additionally, host tracking analysis indicated that ARGs were mainly carried by enterobacteria in the greenhouse soils but actinomyces in the mountain soils. These findings confirmed that some fungicides might serve as the co-selectors of ARGs and elevated their abundance via MGEs-mediated horizontal gene transfer in the greenhouse soils. Image 1 • Fungicides degraded faster in the greenhouse soil (GS) than in the mountain soil (MS). • GS harbored more diverse and abundant ARGs than MS. • Carbendazim could significantly increase the abundance of ARGs in the GS. • Mobile genetic elements were closely associated with the rising of ARGs in the GS. • ARGs were mainly carried by enterobacteria in the GS but actinomyces in the MS. Fungicides elevated the abundance of antibiotic resistome in greenhouse soil. [ABSTRACT FROM AUTHOR]

Livestock manure is a reservoir for antibiotic resistance genes (ARGs), and aerobic composting is used widely for recycling animal manure. This study investigated the effects of adding nano-zerovalent iron (nZVI) at 0, 100, and 1000 mg/kg on the fates of ARGs and mobile genetic elements (MGEs) during swine manure composting. Under nZVI at 100 mg/kg, the relative abundances of sul1 , sul2 , dfrA7 , ermF , and ermX decreased by 33.26–99.31% after composting, and the relative abundances of intI2 and Tn916/1545 decreased by 95.59% and 97.65%, respectively. Most of the ARGs and MGEs co-occurred and they had strong correlations with each other. The bacterial community structure was significantly separated by the composting periods, and they clustered together under different treatments in the same phase. Network analysis showed that Solibacillus , Clostridium_sensu_stricto_1 , Terrisporobacter , Rombou t s ia , Turicibacter , Lactobacillus , Planococcus , Dietzia , and Corynebacterium_1 were common potential hosts of ARGs and MGEs. Redundancy analysis suggested that MGEs had key effects on the variations in the relative abundances of ARGs. Adding 100 mg/kg nZVI could reduce the environmental risk of ARGs by decreasing the abundances of MGEs. Image 1 • Adding 100 mg/kg nZVI most effective at removing ARGs and MGEs. • Most of the ARGs and MGEs co-occurred and they were highly correlated. • Seventeen genera identified as potential host bacteria for ARGs and MGEs. • MGEs responsible for variations in the ARG profiles. Adding 100 mg/kg nZVI led to lower relative abundances of 6/12 ARGs and MGEs were responsible for the changes in ARGs. [ABSTRACT FROM AUTHOR]

Surface waters could be a dominant route by which antibiotic resistance genes (ARGs) are disseminated. In the present study we explored the prevalence and abundance of ARGs [ bla CTX-M-1 , erm (B), sul1 , tet (B), tet (M), and tet (X)], class 1 integron-integrase gene (intI1), and IncP-1 and IncQ-1 plasmids in eight irrigation return flows (IRFs) and a background site (Main Line Canal, MLC) in the Upper Snake Rock watershed in southern Idaho. Grab samples were collected on a monthly basis for a calendar year, which were processed to extract microbial DNA, followed by droplet digital PCR to quantify the gene copies on an absolute (per 100 mL) and relative (per 16S rRNA gene copies) basis. The antibiotic resistance and intI1 genes and IncP-1/IncQ-1 plasmids were recovered at all IRF sampling sites with detections ranging from 55 to 81 out of 81 water sampling events. The bla CTX-M-1 gene was detected the least frequently (68%), while the other genes were detected more frequently (88–100%). All of the genes were also detected at MLC from April to Oct when water was present in the canal. The genes from lowest to greatest relative abundance in the IRFs were: bla CTX-M-1 < erm (B) < tet (B) < IncQ-1 < tet (M) < sul1 < intI1 = IncP-1 < tet (X). When compared to the average annual relative gene abundances in MLC water samples, they were found to be at statistically greater levels (P ≤ 0.008) except that of the IncP-1 and IncQ-1 plasmids (P = 0.8 and 0.08, respectively). The fact that most IRFs contained higher levels than found in the canal water, indicates that IRFs can be a point source of ARGs that ultimately discharge into surface waters. Image 1 • An investigation of antibiotic resistance genes (ARGs) was conducted in an Idaho watershed. • Irrigation return waters contained higher ARG levels than at a background canal site. • Irrigation return flows can be a point source of ARGs and enhance their environmental dissemination. [ABSTRACT FROM AUTHOR]