1. World within world: Intestinal bacteria combining physiological parameters to investigate the response of Metaphire guillelmi to tetracycline stress
- Author
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Feng Hu, Chao Huizhen, Mingming Sun, Mao Ye, and Zheng Xiaoxuan
- Subjects
010504 meteorology & atmospheric sciences ,medicine.drug_class ,Firmicutes ,Tetracycline ,Health, Toxicology and Mutagenesis ,Antibiotics ,010501 environmental sciences ,Toxicology ,01 natural sciences ,Microbiology ,medicine ,Animals ,Soil Pollutants ,Oligochaeta ,Coelomocyte ,0105 earth and related environmental sciences ,biology ,Bacteria ,Planctomycetes ,Tetracycline Resistance ,General Medicine ,biology.organism_classification ,Pollution ,Enzyme assay ,Anti-Bacterial Agents ,Toxicity ,biology.protein ,Proteobacteria ,medicine.drug - Abstract
Due to the abusive usage of antibiotics in animal husbandry, a large amount of residual antibiotics has been released into the environment, therein posing great threat against both environment security and public health. Therefore, it is of great significance to investigate the toxicity of antibiotics on the widely-applied bioindicator-earthworm. In this work, the physiological parameters and the intestinal bacteria community of Metaphire guillelmi were monitored simultaneously to evaluate their sensitivity to the tetracycline (TC) exposure. As expected, the antioxidant enzyme activity and coelomocyte apoptosis acted fairly well as biomarkers for the TC toxicity. In contrast, the intestinal bacteria of Metaphire guillelmi responded varyingly to different TC doses. When TC concentration increased from 0 to 35.7 μg cm−2, the percentage of the Proteobacteria phylum declined significantly from 85.5% to 34.4%, while the proportions of the Firmicutes, Planctomycetes and Atinomycete phyla clearly increased (p
- Published
- 2019