1. As3MT-mediated SAM consumption, which inhibits the methylation of histones and LINE1, is involved in arsenic-induced male reproductive damage
- Author
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Lu Wu, Han Li, Fuping Ye, Yongyue Wei, Wenqi Li, Yuan Xu, Haibo Xia, Jingshu Zhang, Lianxian Guo, Guiwei Zhang, Feng Chen, and Qizhan Liu
- Subjects
Male ,S-Adenosylmethionine ,Health, Toxicology and Mutagenesis ,General Medicine ,DNA ,Methyltransferases ,DNA Methylation ,Toxicology ,Pollution ,Arsenic ,Histones ,Mice ,Vitamin B 12 ,Folic Acid ,Semen ,Arsenic Poisoning ,5-Methylcytosine ,Animals - Abstract
Studies have demonstrated that arsenic (As) induces male reproductive injury, however, the mechanism remains unknown. The high levels of arsenic (3) methyltransferase (As3MT) promote As-induced male reproductive toxicity. For As-exposed mice, the germ cells in seminiferous tubules and sperm quality were reduced. Exposure to As caused lower S-adenosylmethionine (SAM) and 5-methylcytosine (5 mC) levels, histone and DNA hypomethylation, upregulation of long interspersed element class 1 (LINE1, or L1), defective repair of double-strand breaks (DSBs), and the arrest of meiosis, resulting in apoptosis of germ cells and lower litter size. For GC-2spd (GC-2) cells, As induced apoptosis, which was prevented by adding SAM or by reducing the expression of As3MT. The levels of LINE1, affected by SAM content, were involved in As-induced apoptosis. Furthermore, folic acid (FA) and vitamin B12 (VB
- Published
- 2022