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In order to understand the status of Salmonella contamination of feed production chain in Karst rural areas, southwest of China, a total of 1077 feed samples including animal feed materials and feed products were randomly collected from different sectors of feed chain covering feed mills, farms, and feed sales in nine regions of Karst rural areas between 2009 and 2012, to conduct Salmonella test. The different positive rates with Salmonella contamination were detected, the highest was 4.7 % in 2009, the lowest was 0.66 % in 2011, while 4.3 % in 2010, 2.8 % in 2012, respectively. Twelve types of feed including concentrate, complete, self-made, and feed ingredients were inspected. Salmonella contamination mainly concentrated on animal protein material such as meat meal, meat and bone meal, feather meal, blood meal, and fish meal. No Salmonella contamination was detected in feed yeast, microbial protein, rapeseed, and soybean meal. Salmonella contamination existed in each sector of feed production chain. This investigation provided a basic reference for feed production management and quality control in feed production chain in Karst rural areas of China. [ABSTRACT FROM AUTHOR]