Search

Your search keyword '"Suresh, P."' showing total 62 results
Start Over Author "Suresh, P." Journal environmental science & technology
62 results on '"Suresh, P."'

1. High-Resolution Mass Spectrometry for Human Exposomics: Expanding Chemical Space Coverage.

Author

Yunjia Lai, Koelmel, Jeremy P., Walker, Douglas I., Price, Elliott J., Papazian, Stefano, Manz, Katherine E., Castilla-Fernández, Delia, Bowden, John A., Nikiforov, Vladimir, David, Arthur, Bessonneau, Vincent, Amer, Bashar, Seethapathy, Suresh, Xin Hu, Lin, Elizabeth Z., Jbebli, Akrem, McNeil, Brooklynn R., Barupal, Dinesh, Cerasa, Marina, and Hongyu Xie

Published
2024
Full Text
View/download PDF

2. Potential of fluorescence imaging techniques to monitor mutagenic PAH uptake by microalga.

Author

Subashchandrabose, Suresh Ramraj, Krishnan, Kannan, Gratton, Enrico, Megharaj, Mallavarapu, and Naidu, Ravi

Subjects
Chlorella, Dimethylformamide, Benzo(a)pyrene, Water Pollutants, Chemical, Mutagens, Microscopy, Fluorescence, Fluorescence Resonance Energy Transfer, Fluorescence, Microalgae, Environmental Sciences
Abstract

Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), is one of the major environmental pollutants that causes mutagenesis and cancer. BaP has been shown to accumulate in phytoplankton and zooplankton. We have studied the localization and aggregation of BaP in Chlorella sp., a microalga that is one of the primary producers in the food chain, using fluorescence confocal microscopy and fluorescence lifetime imaging microscopy with the phasor approach to characterize the location and the aggregation of BaP in the cell. Our results show that BaP accumulates in the lipid bodies of Chlorella sp. and that there is Förster resonance energy transfer between BaP and photosystems of Chlorella sp., indicating the close proximity of the two molecular systems. The lifetime of BaP fluorescence was measured to be 14 ns in N,N-dimethylformamide, an average of 7 ns in Bold's basal medium, and 8 ns in Chlorella cells. Number and brightness analysis suggests that BaP does not aggregate inside Chlorella sp. (average brightness = 5.330), while it aggregates in the supernatant. In Chlorella grown in sediments spiked with BaP, in 12 h the BaP uptake could be visualized using fluorescence microscopy.

Published
2014

4. MALDI-MS Imaging Analysis of Fungicide Residue Distributions on Wheat Leaf Surfaces

Author

Suresh P. Annangudi, Kyung Myung, Cruz Avila Adame, and Jeffrey R. Gilbert

Subjects
MALDI imaging, Chromatography, Sprayer, fungi, Pesticide application, Analytical chemistry, food and beverages, General Chemistry, Pesticide, Mass spectrometry, Fungicide, chemistry.chemical_compound, chemistry, Azoxystrobin, Environmental Chemistry, Epoxiconazole
Abstract

Improved retention and distribution of agrochemicals on plant surfaces is an important attribute in the biological activity of pesticide. Although retention of agrochemicals on plants after spray application can be quantified using traditional analytical techniques including LC or GC, the spatial distribution of agrochemicals on the plants surfaces has received little attention. Matrix assisted laser desorption/ionization (MALDI) imaging technology has been widely used to determine the distribution of proteins, peptides and metabolites in different tissue sections, but its application to environmental research has been limited. Herein, we probed the potential utility of MALDI imaging in characterizing the distribution of three commercial fungicides on wheat leaf surfaces. Using this MALDI imaging method, we were able to detect 500 ng of epoxiconazole, azoxystrobin, and pyraclostrobin applied in 1 μL drop on the leaf surfaces using MALDI-MS. Subsequent dilutions of pyraclostrobin revealed that the compound can be chemically imaged on the leaf surfaces at levels as low as 60 ng of total applied in the area of 1 μL droplet. After application of epoxiconazole, azoxystrobin, and pyraclostrobin at a field rate of 100 gai/ha in 200 L water using a track sprayer system, residues of these fungicides on the leaf surfaces were sufficiently visualized. These results suggest that MALDI imaging can be used to monitor spatial distribution of agrochemicals on leaf samples after pesticide application.

Published
2015

7. High-Resolution Mass Spectrometry for Human Exposomics: Expanding Chemical Space Coverage

Author

Lai, Yunjia, Koelmel, Jeremy P., Walker, Douglas I., Price, Elliott J., Papazian, Stefano, Manz, Katherine E., Castilla-Fernández, Delia, Bowden, John A., Nikiforov, Vladimir, David, Arthur, Bessonneau, Vincent, Amer, Bashar, Seethapathy, Suresh, Hu, Xin, Lin, Elizabeth Z., Jbebli, Akrem, McNeil, Brooklynn R., Barupal, Dinesh, Cerasa, Marina, Xie, Hongyu, Kalia, Vrinda, Nandakumar, Renu, Singh, Randolph, Tian, Zhenyu, Gao, Peng, Zhao, Yujia, Froment, Jean, Rostkowski, Pawel, Dubey, Saurabh, Coufalíková, Kateřina, Seličová, Hana, Hecht, Helge, Liu, Sheng, Udhani, Hanisha H., Restituito, Sophie, Tchou-Wong, Kam-Meng, Lu, Kun, Martin, Jonathan W., Warth, Benedikt, Godri Pollitt, Krystal J., Klánová, Jana, Fiehn, Oliver, Metz, Thomas O., Pennell, Kurt D., Jones, Dean P., and Miller, Gary W.

Abstract

In the modern “omics” era, measurement of the human exposome is a critical missing link between genetic drivers and disease outcomes. High-resolution mass spectrometry (HRMS), routinely used in proteomics and metabolomics, has emerged as a leading technology to broadly profile chemical exposure agents and related biomolecules for accurate mass measurement, high sensitivity, rapid data acquisition, and increased resolution of chemical space. Non-targeted approaches are increasingly accessible, supporting a shift from conventional hypothesis-driven, quantitation-centric targeted analyses toward data-driven, hypothesis-generating chemical exposome-wide profiling. However, HRMS-based exposomics encounters unique challenges. New analytical and computational infrastructures are needed to expand the analysis coverage through streamlined, scalable, and harmonized workflows and data pipelines that permit longitudinal chemical exposome tracking, retrospective validation, and multi-omics integration for meaningful health-oriented inferences. In this article, we survey the literature on state-of-the-art HRMS-based technologies, review current analytical workflows and informatic pipelines, and provide an up-to-date reference on exposomic approaches for chemists, toxicologists, epidemiologists, care providers, and stakeholders in health sciences and medicine. We propose efforts to benchmark fit-for-purpose platforms for expanding coverage of chemical space, including gas/liquid chromatography–HRMS (GC-HRMS and LC-HRMS), and discuss opportunities, challenges, and strategies to advance the burgeoning field of the exposome.

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results