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Bisphenol A (BPA), one of the endocrine disrupting chemicals, is the object of great concern because of its widespread use throughout the world. In this study, it was aimed to determine the effects of in ovo administrated BPA on the bursa of Fabricius and percentage of acid phosphatase positive lymphocyte in peripheral blood by means of histological and enzyme histochemical methods. For this purpose, 310 fertile eggs of Isa Brown laying parent stock were used. The eggs were divided into 5 groups as control, vehicle control, 50, 100, and 250μg/egg BPA. At days 13, 18, and 21 of incubation, eggs were opened until 10 living embryos were obtained from each group. Tissue samples were taken from the obtained embryos and processed for enzyme histochemical methods in addition to routine histological techniques. It was observed that, in BPA-treated groups, embryonic development of bursa of Fabricius was retarded. It was also indicated that the percentage of peripheral blood ACP-ase positive lymphocytes was significantly decreased. These results suggested that a limited maternal transfer of BPA into the eggs might be lead to immunosuppression in chicks., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)