1. Frog (Pelophylax bergeri, Günther 1986) endocrine disruption assessment: characterization and role of skin poly(ADP-ribose) polymerases
- Author
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Fagr Kh. Abdel-Gawad, Giulia Guerriero, Sofiane Labar, Maria Violetta Brundo, Anna De Maio, D. Rabbito, Samantha Trocchia, Giancarlo Palumbo, Anna Rita Bianchi, Guerriero, Giulia, Brundo, Maria Violetta, Labar, Sofiane, Bianchi, Anna Rita, Trocchia, Samantha, Rabbito, Dea, Palumbo, Giancarlo, Abdel-Gawad, Fagr Kh., and de Maio, Anna
- Subjects
0301 basic medicine ,Amphibian ,Gene isoform ,Male ,medicine.medical_specialty ,Cytoplasm ,Ranidae ,DNA damage ,Poly ADP ribose polymerase ,Health, Toxicology and Mutagenesis ,Poly(ADP-ribose) ,Endocrine disruption assessment ,Pelophylax bergeri ,010501 environmental sciences ,Endocrine Disruptors ,01 natural sciences ,Frog skin ,03 medical and health sciences ,biology.animal ,Internal medicine ,Testis ,medicine ,Environmental Chemistry ,Animals ,Humans ,0105 earth and related environmental sciences ,Skin ,Cell Nucleus ,biology ,Poly(ADP-ribose) polymerase ,General Medicine ,Metals bioaccumulation ,biology.organism_classification ,Molecular biology ,Pollution ,Androgen receptor ,030104 developmental biology ,Endocrinology ,Poikilotherm ,Italy ,Amphibian population decline ,Poly(ADP-ribose) Polymerases ,Frog Skin ,Androgen receptor expression ,Water Pollutants, Chemical ,DNA Damage ,Environmental Monitoring - Abstract
Model of the our research was the adult male amphibian anura, Pelophylax bergeri, poikilotherm species not considered threatened by the IUCN, sampled in representative sites at different degree. In the first phase, a biochemical characterization of the ADP-ribosylation on the skin of barcoded amphibian anura collected from Matese Lake (clean reference site in CE, Italy) was carried out. Two PARP isoforms were evidence: the first of 66 kDa is localized into nucleus and activated by DNA damage; the second of 150 kDa is in cytoplasm, as demonstrated by biochemical and immunohistochemical analysis. Subsequently, the PARP activity, the quantitative expression of androgen receptor gene, and the levels of arsenic and chromium in skin and testis of frog and soil, water, and sediment collected from sites at different degrees of pollution were measured. A significant variation of PARP activity and androgen receptor expression levels was detected in both tissues of barcoded frogs from Sarno and Scafati, along Sarno River (SA, Italy), suggesting that a PARP activation is correlated to pollution and to steroid-regulated physiology disruption.
- Published
- 2016