1. Characteristics of Human Intestinal Acid Sialidase
- Author
-
Scharpé S, Den Tandt Wr, and Adriaenssens K
- Subjects
chemistry.chemical_classification ,Ganglioside ,Adolescent ,Lability ,Albumin ,Infant ,Neuraminidase ,Substrate (chemistry) ,Hydrogen-Ion Concentration ,Sialidase ,Biochemistry ,N-Acetylneuraminic Acid ,Substrate Specificity ,Enzyme ,chemistry ,Child, Preschool ,Intestine, Small ,Mole ,Sialic Acids ,Humans ,Child ,Glycoprotein ,Hymecromone - Abstract
With methylumbelliferyl-N-acetyl-neuraminic acid (MU-NANA) as substrate, acid sialidase was determined in intestinal biopsies of children. The enzyme has an acid pH optimum, a Km value of 4 mmol/l and a pronounced thermal lability which can be partially prevented by the addition of albumin. N-acetyl-neuraminic acid (NANA) and derivatives as well as other glycoprotein and oligosaccharide sialidase substrates inhibit sialidase whereas gangliosides have no effect. This could be an indication that intestinal MU-NANA sialidase is different from ganglioside sialidase as has been reported for many other tissues.
- Published
- 1987