1. One-pot, two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose.
- Author
-
Bawn M, Subrizi F, Lye GJ, Sheppard TD, Hailes HC, and Ward JM
- Subjects
- Arabinose metabolism, Bacterial Proteins metabolism, Biocatalysis, Deinococcus chemistry, Enzyme Stability, Kinetics, Molecular Structure, Monosaccharides metabolism, Pyruvates chemistry, Pyruvates metabolism, Transaminases metabolism, Transketolase metabolism, Arabinose chemistry, Bacterial Proteins chemistry, Deinococcus enzymology, Monosaccharides chemistry, Transaminases chemistry, Transketolase chemistry
- Abstract
The use of biocatalysis for the synthesis of high value added chemical building blocks derived from biomass is becoming an increasingly important application for future sustainable technologies. The synthesis of a higher value chemical from l-arabinose, the predominant monosaccharide obtained from sugar beet pulp, is demonstrated here via a transketolase and transaminase coupled reaction. Thermostable transketolases derived from Deinococcus geothermalis and Deinococcus radiodurans catalysed the synthesis of l-gluco-heptulose from l-arabinose and β-hydroxypyruvate at elevated temperatures with high conversions. β-Hydroxypyruvate, a commercially expensive compound used in the transketolase reaction, was generated in situ from l-serine and α-ketoglutaric acid via a thermostable transaminase, also from Deinococcus geothermalis. The two steps were investigated and implemented in a one-pot system for the sustainable and efficient production of l-gluco-heptulose., (Copyright © 2018. Published by Elsevier Inc.)
- Published
- 2018
- Full Text
- View/download PDF