1. A mechanistic role for the chromatin modulator, NAP1L1, in pancreatic neuroendocrine neoplasm proliferation and metastases
- Author
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Ben Lawrence, Irvin M. Modlin, Mark Kidd, Markus W. Büchler, Daniele Alaimo, Hubertus Schmitz-Winnenthal, Simon Schimmack, and Andrew Taylor
- Subjects
pNETs ,NAP1L1 ,Research ,Proliferation ,Methylation ,Biology ,Promoter methylation ,p57 ,Bioinformatics ,Chromatin ,Pancreatic Neuroendocrine Neoplasm ,Small hairpin RNA ,Gene expression ,Genetics ,Cancer research ,Gene silencing ,Pancreatic neuroendocrine neoplasms ,Molecular Biology ,Chromatin immunoprecipitation - Abstract
The chromatin remodeler NAP1L1, which is upregulated in small intestinal neuroendocrine neoplasms (NENs), has been implicated in cell cycle progression. As p57Kip2 (CDKN1C), a negative regulator of proliferation and a tumor suppressor, is controlled by members of the NAP1 family, we tested the hypothesis that NAP1L1 may have a mechanistic role in regulating pancreatic NEN proliferation through regulation of p57Kip2. NAP1L1 silencing (siRNA and shRNA/lipofectamine approach) decreased proliferation through inhibition of mechanistic (mammalian) target of rapamycin pathway proteins and their phosphorylation (p
- Published
- 2014
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