1. LncRNA Gm12840 mediates WISP1 to regulate ischemia-reperfusion-induced renal fibrosis by sponging miR-677-5p
- Author
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Youling Fan, Jiying Zhong, Jun Zhou, Hongtao Chen, Huan Jing, Simin Tang, Zhenxing Huang, Meijuan Liao, and Sen Lin
- Subjects
Male ,0301 basic medicine ,Cancer Research ,Biology ,Kidney ,CCN Intercellular Signaling Proteins ,Transforming Growth Factor beta1 ,Pathogenesis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,Proto-Oncogene Proteins ,microRNA ,Genetics ,medicine ,Renal fibrosis ,Animals ,Humans ,RNA, Messenger ,Protein kinase B ,Mice, Inbred BALB C ,Acute Kidney Injury ,Fibroblasts ,medicine.disease ,MicroRNAs ,HEK293 Cells ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Reperfusion Injury ,030220 oncology & carcinogenesis ,NIH 3T3 Cells ,Cancer research ,RNA, Long Noncoding ,Signal transduction ,Proto-Oncogene Proteins c-akt ,Signal Transduction ,Kidney disease - Abstract
Aim: We aimed to identify that long noncoding RNAs (lncRNAs) are involved in ischemia-reperfusion (IR)-induced late fibrosis of kidney and may constitute novel therapeutic strategies for acute kidney injury-induced chronic kidney disease. Materials & methods: We performed the mouse model of IR later induced renal fibrosis and analyzed lncRNA profiles using second-generation sequencing during the pathogenesis. Results: The expression levels of 43 lncRNAs and 141 lncRNAs were respectively changed significantly 7 days and 2 weeks after IR treatment. Based on the correlation analysis of the differentially expressed genes, the interaction networks of lncRNAs, miRNAs and mRNA were structured. Conclusion: LncRNA (Gm12840) could act as a sponge for miR-677-5p to mediate fibroblast activation induced by TGF-β1 via the WISP1/PKB (Akt) signaling pathway.
- Published
- 2020
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