1. The methylome and transcriptome of fetal skin: implications for scarless healing.
- Author
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Podolak-Popinigis J, Ronowicz A, Dmochowska M, Jakubiak A, and Sachadyn P
- Subjects
- Animals, Cicatrix genetics, Epigenesis, Genetic, Female, Mice, Inbred C57BL, Pregnancy, Skin injuries, DNA Methylation, Fetus, Prenatal Injuries genetics, Skin metabolism, Transcriptome, Wound Healing genetics
- Abstract
Aim: Fetal skin is known to heal without scarring. In mice, the phenomenon is observed until the 16-17 day of gestation - the day of transition from scarless to normal healing. The study aims to identify key methylome and transcriptome changes following the transition., Materials & Methods: Methylome and transcriptome profiles were analyzed in murine dorsal skin using microarray approach., Results & Conclusion: The genes associated with inflammatory response and hyaluronate degradation showed increased DNA methylation before the transition, while those involved in embryonic morphogenesis, neuron differentiation and synapse functions did so after. A number of the methylome alterations were retained until adulthood and correlated with gene expression, while the functional associations imply that scarless healing depends on epigenetic regulation.
- Published
- 2016
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