1. Methods for analysis of the cancer microenvironment and their potential for disease prediction, monitoring and personalized treatments
- Author
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Irene Weibrecht, Carl-Magnus Clausson, Ola Söderberg, Mats Nilsson, and Ida Grundberg
- Subjects
Cell type ,Population ,Cancer Microenvironment ,Review ,Disease ,Computational biology ,Bioinformatics ,03 medical and health sciences ,0302 clinical medicine ,Diagnosis ,Drug Discovery ,medicine ,education ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,Tumor microenvironment ,business.industry ,Health Policy ,Biochemistry (medical) ,Cancer ,Prognosis ,medicine.disease ,Personalized medicine ,030220 oncology & carcinogenesis ,Cancer cell ,PLA ,business ,Padlock probes - Abstract
A tumor does not consist of a homogenous population of cancer cells. Therefore, to understand cancer, the tumor microenvironment and the interplay between the different cell types present in the tumor has to be taken into account, and how this regulates the growth and survival of the cancer cells. To achieve a full picture of this complex interplay, analysis of tumor tissue should ideally be performed with cellular resolution, providing activity status of individual cells in this heterogeneous population of different cell-types. In addition, in situ analysis provides information on the architecture of the tissue wherein the cancer cells thrive, providing information of the identity of neighboring cells that can be used to understand cell-cell communication. Herein we describe how padlock probes and in situ PLA can be used for visualization of nucleic acids and protein activity, respectively, directly in tissue sections, and their potential future role in personalized medicine.
- Published
- 2012
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