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APPENDIX Protocol for mechanical inoculation of test plants Buffers and chemicals Standard phosphate buffers can be used for inoculation of most viruses and plant species. B Specific scope: b This Standard describes how to perform mechanical inoculation of test plants for detection, propagation and characterisation of plant viruses and viroids. In the past, mechanical inoculation was also used for virus identification, by selecting test plants that could produce a characteristic/specific symptom profile for the virus isolate. [Extracted from the article]

Journal of Plant Pathology 87, 45 - 51. 25 Felix MR & Clara MIE (2002) Two necrovirus isolates with properties of olive latent virus 1 and tobacco necrosis virus from olive in Portugal. Scientific opinion on the risk to plant health posed by Arabis mosaic virus, Raspberry ringspot virus, Strawberry latent ringspot virus and Tomato black ring virus to the EU territory with the identification and evaluation of risk reduction options. B Specific scope: b This Standard describes the production of certified pathogen-tested olive trees and rootstocks. 42 Mathioudakis MM, Saponari M, Hasiowjaroszewska B, Elbeaino T & Koubouris G (2020) Detection of viruses in olive cultivars in Greece, using a rapid and effective RNA extraction method, for certification of virus-tested propagation material. [Extracted from the article]