1. Molecular characterization of a morphologically unusual potato cyst nematode*.
- Author
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Reid, A. and Pickup, J.
- Subjects
- *
GOLDEN nematode , *GLOBODERA , *HETERODERIDAE , *GLOBODERA pallida , *SOIL & civilization , *AGRICULTURAL resources , *CROPS & soils - Abstract
During routine screening of soil samples for potato cyst nematodes, a morphologically variant cyst was detected. The internal transcribed spacer regions of the ribosomal DNA repeat unit from individual juveniles hatched from the cyst were amplified by PCR. The resulting products were digested with a range of restriction enzymes and their RFLP profiles compared with standard samples ofGlobodera rostochiensisandGlobodera pallida. Most of the RFLP profiles yielded patterns similar to those ofG. pallidaalthough some restriction enzymes gave hybrid patterns showing elements of bothG. rostochiensisandG. pallida. Clones of individual ITS regions from a single nematode from the variant cyst displayed either aG. pallidaorG. rostochiensispattern. [ABSTRACT FROM AUTHOR] more...
- Published
- 2005
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