1. Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains
- Author
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Lee G. Elliott, Matthew C. Posewitz, Jonathan E. Meuser, G. Charles Dismukes, Lieve M.L. Laurens, Randor Radakovits, David J. Vinyard, Robert E. Jinkerson, and Victoria H. Work
- Subjects
Chlorophyll ,Chromatography, Gas ,Anabolism ,Starch ,Nitrogen ,Mutant ,Chlamydomonas reinhardtii ,Cell Count ,Carbohydrate metabolism ,Acetates ,Microbiology ,chemistry.chemical_compound ,Isoamylase ,Photosynthesis ,Molecular Biology ,chemistry.chemical_classification ,Flame Ionization ,biology ,Fatty Acids ,Genetic Complementation Test ,Fatty acid ,Lipid metabolism ,General Medicine ,Articles ,biology.organism_classification ,Lipid Metabolism ,Oxygen ,chemistry ,Biochemistry ,Microscopy, Fluorescence ,Mutation ,Carbohydrate Metabolism - Abstract
The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7 - 10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7 - 10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7 - 10 mutants increases the accumulation of lipids on a cellular basis during nitrogen deprivation relative to that in the CC124 control as determined by conversion to fatty acid methyl esters. However, this increased level of lipid accumulation is energetically insufficient to completely offset the loss of cellular starch that is synthesized by CC124 during nitrogen deprivation. We therefore investigated acetate utilization and O 2 evolution to obtain further insights into the physiological adjustments utilized by the two starchless mutants in the absence of starch synthesis. The results demonstrate that both starchless mutants metabolize less acetate and have more severely attenuated levels of photosynthetic O 2 evolution than CC124, indicating that a decrease in overall anabolic processes is a significant physiological response in the starchless mutants during nitrogen deprivation. Interestingly, two independent sta7 - 10 : STA7 complemented strains exhibited significantly greater quantities of cellular starch and lipid than CC124 during acclimation to nitrogen deprivation. Moreover, the complemented strains synthesized significant quantities of starch even when cultured in nutrient-replete medium.
- Published
- 2010