Search

Your search keyword '"Ann Brinkmalm"' showing total 2 results
Start Over Author "Ann Brinkmalm" Journal eupa open proteomics
2 results on '"Ann Brinkmalm"'

1. Quantification of total apolipoprotein E and its specific isoforms in cerebrospinal fluid and blood in Alzheimer’s disease and other neurodegenerative diseases

Author

Melinda Rezeli, Henrik Zetterberg, Kaj Blennow, Ann Brinkmalm, Thomas Laurell, Oskar Hansson, and György Marko-Varga

Subjects
CSF biomarkers, Alzheimer’s disease, SRM, Protein isoforms, apoE, Genetics, QH426-470
Abstract

A targeted mass spectrometric assay was developed for identification and quantification of apoE isoforms (apoE2, E3 and E4), and it was utilized for screening of samples from AD patients (n = 39) and patients with other neurodegenerative disorders (n = 38). The assay showed good linearity with LOQ corresponds to total apoE concentration of 0.8 and 40 ng/mL in CSF and plasma/serum, respectively. We identified apoE phenotypes with 100% accuracy in clinical samples. We found strong association between genotypes of the individuals and their apoE levels in blood; ϵ4 allele carriers had significantly lower apoE levels in blood than non-carriers.

Published
2015
Full Text
View/download PDF

2. Quantification of total apolipoprotein E and its specific isoforms in cerebrospinal fluid and blood in Alzheimer’s disease and other neurodegenerative diseases

Author

György Marko-Varga, Henrik Zetterberg, Kaj Blennow, Thomas Laurell, Oskar Hansson, Ann Brinkmalm, and Melinda Rezeli

Subjects
Gene isoform, Apolipoprotein E, SRM, medicine.medical_specialty, lcsh:QH426-470, Disease, Biology, Biochemistry, Phenotype, apoE, lcsh:Genetics, Endocrinology, Cerebrospinal fluid, Internal medicine, Immunology, Genotype, medicine, lipids (amino acids, peptides, and proteins), Total apolipoprotein E, Allele, CSF biomarkers, Alzheimer’s disease, Protein isoforms
Abstract

A targeted mass spectrometric assay was developed for identification and quantification of apoE isoforms (apoE2, E3 and E4), and it was utilized for screening of samples from AD patients ( n = 39) and patients with other neurodegenerative disorders ( n = 38). The assay showed good linearity with LOQ corresponds to total apoE concentration of 0.8 and 40 ng/mL in CSF and plasma/serum, respectively. We identified apoE phenotypes with 100% accuracy in clinical samples. We found strong association between genotypes of the individuals and their apoE levels in blood; ϵ4 allele carriers had significantly lower apoE levels in blood than non-carriers.

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results