1. Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on the chromosome P4 using PCR-based markers useful for breeding programmes
- Author
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Aleksandra Korzeniewska, Alain Palloix, Piotr Gawroński, Hanna Bolibok-Brągoszewska, Cezary Waszczak, Iwona Stępień, Grzegorz Bartoszewski, Véronique Lefebvre, Katarzyna Niemirowicz-Szczytt, Warsaw University of Life Sciences (SGGW), Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), and Polish Ministry of Agriculture and Rural Development
- Subjects
0106 biological sciences ,Sterility ,[SDV]Life Sciences [q-bio] ,LOCI ,Genetic mapping ,Locus (genetics) ,Plant Science ,Horticulture ,Biology ,ms8 ,01 natural sciences ,03 medical and health sciences ,Gene mapping ,Pepper ,SOLANACEAE ,Genetics ,QTL ANALYSIS ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,POLYGENIC RESISTANCE ,Genic male sterility ,Gene ,PCR-based markers ,030304 developmental biology ,Molecular breeding ,0303 health sciences ,IDENTIFICATION ,Gene map ,food and beverages ,Chromosome P4 ,Genetic marker ,MAP ,TOMATO ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
The nuclear male sterility gene ms8 is expected to facilitate the production of sweet pepper (Capsicum annuum L.) hybrids as it provides means for hybridization without the labor-intensive hand emasculation of female inbred lines. The development of molecular markers linked to ms8 locus will help the breeding practice for the selection of hybrid parental lines. In this study, F2 population resulting from a cross between the sweet pepper male sterile line 320 and the male fertile variety Elf was used to identify DNA markers linked to the ms8 locus. With the use of RAPD–BSA technique, seven markers linked to the ms8 locus were found. Four of them were converted into SCAR markers. In addition, two COSII/CAPS markers linked to the ms8 locus were identified. Comparative mapping with reference pepper maps indicated that the ms8 locus is located on the lower arm of the pepper chromosome P4. Identified markers are useful for molecular breeding, however, at present markers tightly linked to ms8 locus are still lacking. Identification of molecular markers linked to the ms8 locus and determination of its chromosomal localization are useful for fine mapping and also provide the perspective for ms8 gene cloning.
- Published
- 2012
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