1. Three AFLP markers tightly linked to the genic male sterility ms 3 gene in chili pepper (Capsicum annuum L.) and conversion to a CAPS marker
- Author
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Jung-Heon Han, Jae Bok Yoon, Sang Hoon Kim, Jundae Lee, Won Phil Lee, and Hyo Guen Park
- Subjects
Genetics ,education.field_of_study ,Sterility ,Population ,Bulked segregant analysis ,food and beverages ,Locus (genetics) ,Plant Science ,Horticulture ,Biology ,Genetic marker ,Pepper ,Cleaved amplified polymorphic sequence ,Amplified fragment length polymorphism ,education ,Agronomy and Crop Science - Abstract
Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). We developed DNA markers linked to the GMS ms 3 gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc276, Eagc/Mctt178, and Ecag/Mtgc204) were identified as tightly linked to the ms 3 locus. Among them, we converted the AFLP marker Ecag/Mtgc204 to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using the GMS system.
- Published
- 2009
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