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The oil accumulation in the developing soybean seed has been shown to be a dynamic process with different rates and activities at different phases affected by both genotype and environment. The objective of the present study was to investigate additive, epistatic and quantitative trait loci (QTL) × environment interaction (QE) effects of the QTL controlling oil filling rate in soybean seed. A total of 143 recombinant inbred lines (RILs) derived from the cross of Charleston and Dongnong 594 were used in this study to obtain 2 years of field data (2004 and 2005). A total of 26 QTL with significantly unconditional and conditional additive ( a) effect and/or additive × environment interaction ( ae) effect at different filling stages were identified on 14 linkage groups. Among the QTL with significant a effects, 18 QTL showed positive effects and 6 QTL had negative effects on seed filling rate of oil content during seed development. A total of 29 epistatic pairwise QTL underlying seed filling rate were identified at different filling stages. About 28 pairs of the QTL showed additive × additive epistatic ( aa) effects and 14 pairs of the QTL showed aa × environment interaction ( aae) effects at different filling stages. QTL with aa and aae (additive × additive × environment) effects appeared to vary at different filling stages. Our results demonstrated that oil filling rate in soybean seed were under genetic, developmental and environmental control. [ABSTRACT FROM AUTHOR]

The oil accumulation in the developing soybean seed has been shown to be a dynamic process with different rates and activities at different phases. Transcript abundance measurements showed oil accumulation was affected by multiple genetic loci that were active in different seed filling phases (Schmutz et al., Nature 463:178-183, 2010). The objective of this study was to identify quantitative trait loci (QTL) controlling oil accumulation during seed development. One hundred forty-three recombinant inbred lines (RILs) derived from the cross of Charleston and Dongnong 594 were used to obtain two year field data in 2004 and 2005. Thirty-four unconditional QTL underlying oil filling rate at the different developmental stages were mapped onto fourteen linkage groups. The phenotypic variation on oil filling rate explained by these unconditional QTL ranged from 4.9 to 34.8%. Thirty-five conditional QTL underlying the oil filling rate of seed were mapped onto fifteen linkage groups. The phenotypic variation explained by these conditional QTL ranged from 5.4 to 26.0%. The locations, numbers, genetic effects and types of QTL for oil filling rate were different at each seed developmental stage. Genotype by environment (G × E) interaction effects among some QTL underlying oil filling rate were observed. In addition, several genomic regions affected seed oil filling rate were detected. Therefore, oil QTL detected with data from harvest time may miss or erroneously locate the key loci. [ABSTRACT FROM AUTHOR]

Seed protein content at the harvest stage is the sum of protein accumulation during seed filling. The aim of our investigation was to identify loci underlying the filling rate of seed protein at different developmental stages. To this end, we used 143 recombinant inbred lines (RILs) derived from the cross of soybean cultivars ‘Charleston’ and ‘Dongnong 594’ and composite interval mapping with a mixed genetic model. The genotype × environment interactions of the quantitative trait loci (QTL) were also evaluated. Thirty-nine unconditional QTL underlying the filling rate of seed protein at five developmental stages were mapped onto 14 linkage groups. The proportion of phenotypic variation explained by these QTL ranged from 4.88 to 26.05%. Thirty-eight conditional QTL underlying the filling rate of seed protein were mapped onto 16 linkage groups. The proportion of phenotypic variation explained by these QTL ranged from 1.87 to 31.34%. The numbers and types of QTL and their genetic effects on the filling rate of seed protein were different at each developmental stage. A G × E interaction effect was observed for some QTL. [ABSTRACT FROM AUTHOR]