1. Critical stresses for cancer cell detachment in microchannels
- Author
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Cécile Couzon, Claude Verdier, Alain Duperray, Verdier, Claude, Laboratoire de Spectrométrie Physique (LSP), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), INSERM U823, équipe 8 (Immunologie Analytique des Pathologies Chroniques), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), and Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
Materials science ,MESH: Cell Line, Tumor ,Critical stress ,Microfluidics ,Biophysics ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Models, Biological ,MESH: Cell Adhesion ,Focal adhesion ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,MESH: Computer Simulation ,Cell Movement ,[SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] ,Cell Line, Tumor ,Shear stress ,Cell Adhesion ,Humans ,Computer Simulation ,Composite material ,[PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] ,MESH: Cell Movement ,Shearing (physics) ,Focal Adhesions ,MESH: Humans ,MESH: Stress, Mechanical ,critical stress ,[PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] ,MESH: Models, Biological ,MESH: Focal Adhesions ,[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] ,General Medicine ,MESH: Urinary Bladder Neoplasms ,Shear (geology) ,Urinary Bladder Neoplasms ,cancer cells ,MESH: Shear Strength ,Adhesive ,Stress, Mechanical ,Contact area ,Shear Strength - Abstract
International audience; We present experiments involving cancer cells adhering to microchannels, subjected to increasing shear stresses (0.1-30 Pa). Morphological studies were carried out at different shear stresses. Cells exhibit spreading patterns similar to those observed under static conditions, as long as the shear stress is not too high. At critical wall shear stresses (around 2-5 Pa), cell-substrate contact area decreases until detachment at the larger stresses. Critical shear stresses are found to be lower for higher confinements (i.e. smaller cell height to channel height ratio). Fluorescent techniques were used to locate focal adhesions (typically 1 lm(2) in size) under various shearing conditions, showing that cells increase the number of focal contacts in the region facing the flow. To analyze such data, we propose a model to determine the critical stress, resulting from the competition between hydrodynamic forces and the adhesive cell resistance. With this model, typical adhesive stresses exerted at each focal contact can be determined and are in agreement with previous works.
- Published
- 2009