1. 57 Fe enrichment in mice for β-thalassaemia studies via Mössbauer spectroscopy of blood samples.
- Author
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Charitou G, Tsertos C, Parpottas Y, Kleanthous M, Lederer CW, and Phylactides M
- Subjects
- Animals, Intestinal Absorption, Mice, Mice, Inbred C57BL, Iron Isotopes metabolism, Iron Isotopes pharmacology, Spectroscopy, Mossbauer, beta-Thalassemia blood, beta-Thalassemia metabolism
- Abstract
In this work, wild-type and heterozygous β-thalassaemic mice were enriched with
57 Fe via gastrointestinal absorption to characterize in greater detail the iron complexes then identifiable via Mössbauer spectroscopy. The57 Fe enrichment method was validated and Mössbauer spectra were obtained at 80 K from blood samples from wild-type and β-thalassaemic mice at 1, 3, 6, and 9 months of age. As expected, the haemoglobin levels of the thalassaemic mice were lower than from normal mice, indicating anaemia. Furthermore, significant amounts of ferritin-like iron were observed in the thalassaemic mice samples, which decreased with mouse age, reflecting the pattern of reticulocyte count reduction reported in the literature.- Published
- 2019
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