1. Structural investigations on the 2-keto-3-deoxyoctonate region of lipopolysaccharides
- Author
-
Otto Westphal, Wulf Dröge, Otto Lüderitz, and Volker Lehmann
- Subjects
Boron Compounds ,Electrophoresis ,Lipopolysaccharides ,Paper ,Chromatography, Gas ,Glycoside Hydrolases ,Biochemistry ,Phosphates ,Lipid A ,chemistry.chemical_compound ,Glycolipid ,Phosphorylethanolamine ,Cell Wall ,Salmonella ,Glycoside hydrolase ,Trisaccharide ,chemistry.chemical_classification ,Periodic Acid ,Polysaccharides, Bacterial ,Periodic acid ,Periodate ,Heptoses ,Amino Alcohols ,Semicarbazides ,carbohydrates (lipids) ,chemistry ,Barbiturates ,Mutation ,lipids (amino acids, peptides, and proteins) ,Acid hydrolysis ,Chromatography, Thin Layer ,Oxidation-Reduction - Abstract
Mild acid hydrolysis of the glycolipid derived from a Salmonella Rd2 mutant led to the liberation of three major split products which, after separation by electrophoresis, were identified as free 2-keto-3-deoxyoctonate (KDO), KDO-7-phosphorylethanolamine, and l-glycero-α-d-mannoheptosyl-1,5-KDO. By the use of various methods of KDO determinations, and by periodate oxidation performed on the glycolipid, results were obtained which indicate that the bridge between the polysaccharide portion of lipopolysaccharides and lipid A is formed by a branched KDO trisaccharide whose KDO side chain is randomly substituted by phosphorylethanolamine according to the formula —Hep-1,5-KDO-KDO-lipid A | KDO-(phosphorylethanolamine) Analogous results were obtained with P-lipopolysaccharides of other Salmonella mutants.
- Published
- 1970