1. Nucleic acid sequence of the region of the genome encoding capsid protein VP1 of neurovirulent and attenuated type 3 polioviruses.
- Author
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Stanway G, Cann AJ, Hauptmann R, Mountford RC, Clarke LD, Reeve P, Minor PD, Schild GC, and Almond JW
- Subjects
- Base Sequence, Cloning, Molecular, DNA, Viral isolation & purification, Humans, Poliovirus pathogenicity, RNA, Viral isolation & purification, Virulence, Capsid genetics, Poliovirus genetics, Poliovirus Vaccine, Oral, Vaccines, Attenuated
- Abstract
Three closely related strains of poliovirus type 3 have been used to study the molecular basis of attenuation in the currently used Sabin vaccine of this serotype. Plaque-purified derivatives of these strains possess closely similar serological and biochemical properties yet differ markedly in neurovirulence for monkeys. Molecular cloning via an RNA . cDNA method has facilitated comparative nucleotide sequencing. Initial efforts have concentrated on the region of the genome encoding VP1. Only minor structural differences between neurovirulent and attenuated type 3 strains were detected, in contrast to the major differences observed between the vaccine strains of poliovirus type 1 and its virulent precursor P1/Mahoney. These observations suggest that the molecular basis of attenuation of type 3 Sabin vaccine virus does not involve the VP1 polypeptide and, therefore, that mutations conferring the attenuated phenotype probably lie elsewhere in the genome.
- Published
- 1983
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